RNase-Free DNase I Kit

Features and Benefits

Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits
Also includes protocol for digestion in-solution followed by RNA Clean-Up
Guaranteed RNase-Free
Includes Enzyme Incubation Buffer
Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)

Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.

Details

Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.

Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.

Component	Cat. 25710 (50 rxns)	Cat. 25720 (200 rxns)
DNase I	1 vial / 1,600 units	4 vials (1,600 units/vial)
Buffer DR	1 mL	4 x 1 mL
Enzyme Incubation Buffer	6 mL	4 x 6 mL
Product Insert	1	1

Documentation

PROTOCOLS

(25710) RNase-Free DNase I Kit - Protocol (50 reactions)

(25720) RNase-Free DNase I Kit - Protocol (200 reactions)

SAFETY DATA SHEETS

25710 - RNase-Free DNase I Kit - SDS

25720 - RNase-Free DNase I Kit - SDS

Citations

Title	Selection of Suitable Internal Control Genes for Accurate Normalization of Real-Time Quantitative PCR Data of Buffalo (Bubalus bubalis) Blastocysts Produced by SCNT and IVF
Citation	Cellular Reprogramming 2023.
Authors	Sood, T. J., Lagah, S. V., Sharma, A., Singla, S. K., Mukesh, M., Chauhan, M. S., ... & Palta, P. (2017).

Title	Sterile inflammation via TRPM8 RNA-dependent TLR3-NF-kB/IRF3 activation promotes antitumor immunity in prostate cancer
Citation	The EMBO Journal 2023.
Authors	Alessandro Alaimo 1,17?, Sacha Genovesi 1 , Nicole Annesi1 , Dario De Felice 1 , Saurav Subedi2, Alice Macchia1 , Federico La Manna2 , Yari Ciani1 , Federico Vannuccini 1 , Vera Mugoni1 Michela Notarangelo 1 , Michela Libergoli 1 , Francesca Broso 1 , Riccardo Taulli3,4, Ugo Ala 5 Aurora Savino6 , Martina Cortese 1 , Somayeh Mirzaaghaei 6,7, Valeria Poli 6,7, Ian Marc Bonapace8 Mauro Giulio Papotti9 , Luca Molinaro9 , Claudio Doglioni10, Orazio Caffo11, Adriano Anesi12, Michael Nagler13, Giovanni Bertalot 14,15, Francesco Giuseppe Carbone14, Mattia Barbareschi 14,15, Umberto Basso16, Erik Dassi1 , Massimo Pizzato1 , Alessandro Romanel 1 , Francesca Demichelis1 Marianna Kruithof-de Julio2,13 & Andrea Lunardi

Title	The central anorexigenic mechanism of amylin in Japanese quail (Coturnix japonica) involves pro-opiomelanocortin, calcitonin receptor, and the arcuate nucleus of the hypothalamus
Citation	Comparative Biochemistry and Physiology 2023.
Authors	Yuan, J., Gilbert, E. R., & Cline, M. A. (2017).

Title	the Molecular and Genetic Basis of Repeatable Coevolution between Escherchia coli and Bacteriophage T3 in a Laboratory Microcosm
Citation	PLoS One 2023.
Authors	EB Perry, JE Barrick, BJM Bohannan

Title	The Response of microRNAs to Solar UVR in Skin-Resident Melanocytes Differs between Melanoma Patients and Healthy Persons
Citation	PLoS One 2023.
Authors	Sha J, Gastman BR, Morris N, Mesinkovska NA, Baron ED, Cooper KD, et al.