Intact Exosome Purification
- Purification and enrichment of intact plasma/serum, urine, cell culture media and saliva exosomes
- Purified exosomes for use in functional studies prepared in 30 minutes
- Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
- No time-consuming ultracentrifugation, filtration or special syringes required
- No precipitation reagents, overnight incubation, protease or coagulant treatments required
- Purified exosomes are free from any protein-bound cell-free circulating RNA
- Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
- Purification is based on Norgen's proprietary Silicon Carbide resin matrix
Intact Exosome Purification and Exosomal RNA Isolation
- Purification and enrichment of intact exosomes from plasma/serum, urine and cell culture media followed by subsequent exosomal RNA isolation from the purified intact exosomes
- Purified exosomes for use in functional studies prepared in 30 minutes
- Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
- Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
- Purify high-quality RNA in 15-20 minutes
- Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL
- The purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
- The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Intact Exosome Purification, Exosomal RNA and Free-Circulating RNA Isolation
- Purification and enrichment of intact exosomes from plasma/serum and urine followed by subsequent RNA isolation from both the purified intact exosomes and the circulating cell-free RNA
- Purified exosomes for use in functional studies prepared in 30 minutes
- Purify high-quality exosomal and cell-free circulating RNA (cfc-RNA) in 15-20 minutes
- Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
- The purified exosomes are free from any protein-bound cell-free circulating RNA
- Sequential isolation of all sizes of exosomal/extracellular vesicle RNA and cfc-RNA, including microRNA in two separate fractions irrespective of size or GC content, without bias
- Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL
- The purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
- The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
RNA Isolation from Purified Intact Exosomes
- Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA, from previously purified exosomes
- Bind and elute all RNA irrespective of size or GC content, without bias
- No phenol extractions, Proteinase K treatment or carrier RNA required
- Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL
- Purify high-quality RNA in 15-20 minutes
- Compatible with exosome and extracellular vesicles purified using Norgen's exosome purification technology or using any other exosome purification technology such as ultracentrifugation, Exoquick, filtration or others
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Exosome Depletion
- Efficient depletion of cow's exosomes from Fetal Bovine Serum or any other serum source prior to use as a growth supplement in culture medium
- Deplete exosome-sized vesicles from versatile serum volumes of up to 280 mL
- No protease treatment required
- No time-consuming ultracentrifugation
- No precipitation reagents or overnight incubation required
- Depleted FBS has no detectable cow's miRNA
- The depleted FBS provides the same cellular growth rates as the standard FBS
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Exosome Labeling and Cleaning Kit
- Allows for efficient labelling of exsosomes with very low levels of background
- Allows for labelling of exosomes isolated using various methods, including Norgen Biotek Corp. proprietary kits, ultracentrifugation and precipitation reagents
- Convenient & fast protocol, which includes both labelling and cleaning procedures
- Excitation at 590 nm/Emission at 617 nm