Inclusion Body Protein Purification
- Solubilize and purify inclusion bodies from bacterial cell cultures using Norgen’s unique SiC spin column technology
- Solubilize and purify inclusion bodies from bacterial cell cultures using Norgen’s unique SiC spin column technology
- Rapid purification, independent of affinity tag utilized
- No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
- Versatile sample input volume
- Intact, functional proteins are ready for downstream applications including SDS-PAGE, 2DE, WB, MS analysis, and in vitro applications
Protein Clean-Up, Concentration and Endotoxin Removal
- Simultaneously clean-up and concentrate total isolated proteins using Norgen’s proprietary resin matrix
- Rapidly remove salts, detergents, and endotoxins, without buffer exchange
- Versatile sample input volume
- No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
- >95% protein recovery
- Intact, functional proteins are ready for downstream applications including SDS-PAGE, 2D gels, Western Blotting, whole protein mass spectrometry, and protein microarrays
Urine Protein Concentration
- Simultaneously clean-up and concentrate total urinary proteins using Norgen’s proprietary resin matrix
- Rapidly remove salts and urea without buffer exchange
- Versatile sample input volume
- No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
- Intact, functional proteins are ready for downstream applications including SDS-PAGE, 2D gels, Western Blotting, whole protein mass spectrometry, and protein microarrays
Depletion of Abundant Serum Proteins
- Deplete five highly abundant proteins from serum and plasma by 50-90% using Norgen’s proprietary resin matrix
- Improve detection of low-abundance proteins for biomarker discovery
- No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
- Rapid, convenient, and cost-effective
- Intact, functional proteins are ready for downstream applications including SDS-PAGE, 2D gels, Western Blotting, whole protein mass spectrometry, and protein microarrays
On-Column Proteolytic Digestion
- Simultaneously trypsinize proteins and concentrate peptides using Norgen’s proprietary resin matrix
- Rapid, convenient, and efficient/complete digestion
- Intact, functional proteins are ready for downstream applications including SDS-PAGE, 2D gels, Western Blotting, whole protein mass spectrometry, and protein microarrays
