Efficient Recovery of Large DNA Fragments. The efficient recovery of Norgen's DNA Gel Extraction Kit is illustrated by purification of 1.5 µg of a 3,700 bp fragment from a 0.9% agarose gel using Norgen's DNA Gel Extraction Kit (Lanes 5-7) and a competitor's kit (Lanes 2-4). Eluted DNA was resolved on a 1X TAE, 0.9% agarose gel. Lane 1 indicates 300 ng (20%) of the input amount, while lanes 2-7 contain 10 µL (20%) of the 50 µL eluted amount. Norgen's kit shows a higher recovery than the competitors kit.

High Yield Purification. The high yield of Norgen's PCR Purification Kit is illustrated by purifying 3.5 µg of a 500 bp PCR-amplified product from a reaction mixture and comparing the recovery with a competitor. The purifications were performed in triplicate. DNA recovery was quantified by running 350 ng (1/10th) of the input and 5 µL (1/10th) of 50 µL DNA elution on a 1X TAE, 1% agarose gel followed by densitometry. The average yield for each kit is shown in Figure 1. Norgen's kit shows a significantly higher recovery than the competitor's kit.

Concentration of RNA prior to Next Generation Sequencing (NGS) applications. Total RNA was purified from 200 µL of plasma collected on EDTA blood tubes using Norgen's Total RNA Purification Kit (Cat. 17200) and eluted in 50 µL of elution solution. The same RNA was also concentrated two-fold using the Micro-Elute RNA Column by eluting in 25 µL of elution solution. Five microliters of both the RNA without additional concentration and the 2X concentrated RNA were used as inputs to generate RNA libraries (using the NEBNext® Small RNA Library Prep Set for Illumina® and following manufacturer’s instructions) for small RNA NGS on the MiSeq (Illumina) platform. A) The prepared small RNA libraries were visualized on a 6% TBE polyacrylamide gel, where the library prepared with 2X concentrated RNA contained more ligated/indexed miRNA cDNA (147-160 bp) products than the library prepared using the RNA without concentration. B) The cDNA was extracted from excised gel bands and interrogated using the Agilent 2100 Bioanalyzer (High Sensitivity DNA Assay). As would be expected based on input, the small RNA library prepared with the 2X concentrated RNA sample was approximately two times more concentrated than the library prepared with RNA without prior concentration (39.8 vs 21.2 nM, respectively).

High Recoveries and Efficient Endotoxin Removal. High recoveries of DNA can be seen when using the CleanAll kit, and these high recoveries are not compromised even during endotoxin removal. In Panel A, 1 µg of a PCR product was cleaned using the CleanAll DNA/RNA Clean-Up and Concentration Micro Kit as well as Norgen's PCR Purification Kit. More than 0.9 µg of the DNA was recovered from both kits with a comparable percent recovery of over 90%. In Panel B, 10 µg of plasmid DNA preparation was cleaned from endotoxin by using the CleanAll DNA/RNA Kit. Endotoxin levels of the plasmid DNA sample were reduced from 0.75 EU/µg plasmid DNA to less 0.05 EU/µg plasmid DNA. Approximately 95% of the endotoxin units in the DNA sample were removed using the kit, therefore the kit allows for high recoveries coupled with exceptional cleanup.

epresentative Recovery and Salt Reduction using the ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit.To investigate salt removal using the ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit, 50 µg samples of BSA were prepared in 1 mL samples of sodium acetate and were spiked with 300 ppm of MgCl2, NaCl, KCl, CaCl2 or LiCl. The salt was removed using the provided protocol, and the proteins were eluted into 2 separate 50 µL elutions of 250 mM ammonium hydroxide. Ammonium hydroxide is not the provided elution buffer, but was required to allow for the analysis of the amount of salt in the elutions. The 2 elutions were combined for analysis. The amount of BSA recovery was determined using the BioRad Protein Assay, and the amounts can be seen in the table above. The BSA recoveries were lower than expected due to the fact that an alternate Elution Solution was used for analysis that was not optimized for recovery. The amount of salt present in the elutions was determined by ICP/MS designed for elemental analysis. As it can be seen, salt removal was up to 99.7% using this kit.