Features and Benefits

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Ten discrete fragments from 100 bp to 1000 bp
• Higher intensity reference band at 500 bp

The Norgen PCRSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCRSizer contains ten discrete fragments ranging from 100 bp to 1000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for PCR product size confirmation.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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Figure 1:  PCR Sizer 100 bp DNA Ladder

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PCR Sizer 100 bp DNA Ladder (Cat# 11400) - 100 loads

Ladder Properties:
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference

 

Fragment	Size (bp)	Mass (ng)
1	1000	78
2	900	70
3	800	62
4	700	55
5	600	47
6	500	78
7	400	31
8	300	24
9	200	31
10	100	24

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

 

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Documentation

PROTOCOLS

11400 - Product Insert

APPLICATION NOTES

DNA Quantification by Gel Densitometry with Norgen DNA Ladders

SAFETY DATA SHEETS

11400 - PCR Sizer 100 BP DNA Ladder - SDS

Citations

Title	Standardization of a molecular method for epidemiologic identification of Leishmania strains
Citation	Genet. Mol. Res 2023.
Authors	Rocha, R. F., Menezes, E. V., Xavier, A. R. E. O., Royo, V. A., Oliveira, D. A., Júnior, A. F. M., ... & Michalsky, E. M