Figure 1. Detergent-free Proteins Isolated from E. coli, Yeast, HeLa Cells and Kidney Tissue. Total proteins were isolated from 2 x 10⁹ E. coli cells, 2 x 10⁷ yeast cells, 1 million HeLa cells and 15 mg of Chinese hamster kidney tissue in duplicate using Norgen’s Detergent-Free Total Protein Isolation Kit. The total proteins were analyzed by running aliquots on 12% SDS-PAGE gels. Panel A corresponds to 10 µL of the 40 µL recovered proteins from E. coli., Panel B corresponds to 10 µL of the 40 µL recovered proteins from yeast, Panel C corresponds to 12 µL of the 60 µL recovered proteins from the HeLa cells, while Panel D corresponds to 2 µL of the 60 µL of recovered proteins from the kidney tissue. The proteins are intact and of the highest quality, and can be used in a number of different downstream applications.