Name: Plasma/Serum cfc-RNA Advanced Purification Kit
Brand: Norgen Biotek Corp.
SKU: 68200
Price: 549.000000 USD
Availability: InStock

Supporting Data

Figure 1. A representative Bioanalyzer RNA Pico Chip trace showing the quality/quantity of the RNA purified from 4 mL K2 EDTA plasma using A) Norgen’s Plasma/Serum cfc-RNA Advanced Purification Kit (Automated Isolation), B) Norgen’s Plasma/Serum cfc-RNA Advanced Purification Kit (Manual Isolation), C) Competitor Q's cfc-DNA/RNA Kit and D) Competitor Q's Circulating Nucleic Acid Kit. All purification methods showed the correct cf-RNA size on the bioanalyzer trace however the amount of the cf-RNA recovered using Norgen’s technology (as can be noticed by the height of the cf-RNA peak) was significantly higher than that for the cf-RNA purified using the silica-based technology.

Figure 2. rt-qPCR amplification of miR-21 and miR-16 from RNA purified from K2EDTA plasma using different cfc-RNA purification methods. Norgen’s SiC technology, manual and automated procedure, was superior as compared to the amplification of the same miRNA targets amplified from RNA purified from K2EDTA plasma using the Silica-based technology represented by Qiagen’s purification kits.

Kit Specifications
Minimum Plasma/Serum Input	1 mL
Maximum Plasma/Serum Input	6 mL
Size of cfc-RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	25-50 µL
Time to Complete 10 Purifications	45 minutes
Average Yields*	Variable depending on specimen

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common RNA Quantification Methods.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Kit Components	Cat. 68200 (50 preps)
Binding Buffer A	20 mL
Proteinase K	6.5 mL
Slurry E	12.5 mL
Lysis Buffer A	130 mL
Wash Solution A	38 mL
Elution Buffer C	2 x 8 mL
Elution Solution A	20 mL
Micro Spin Columns	50
Mini Filter Spin Columns	50
Collection Tubes	100
Elution Tubes (1.7 mL)	100
Product Insert	1

Documentation

PROTOCOLS

(68200) Plasma/Serum cfc-RNA Advanced Purification Kit - Protocol (50 Preps)

SAFETY DATA SHEETS

68200 - Plasma/Serum cfc-RNA Advanced Purification Kit - SDS

RELATED BLOGS

FAQs

What if a variable speed centrifuge is not available and the speed differs from the recommended?

A fixed speed centrifuge can be used, however reduced yields may be observed.

At what temperature should I centrifuge my samples?

All centrifugation steps are performed at room temperature. Centrifugation at 4°C will not adversely affect kit performance.

What if I added more or less of the specified reagents' volume?

Adding more or less than the specified volumes may reduce both the quality and the quantity of the purified RNA. Eluting your RNA in high volumes will increase the yield but will lower the concentration. Eluting in small volumes will increase the concentration but will lower the overall yield.

What If I forgot to do a dry spin before my final elution step?

Your purified RNA will be contaminated with the Wash Solution A. This may reduce the quality of your purified RNA and will interfere with your downstream applications.

Can I perform a second elution?

Yes, but it is recommended that the 2nd elution be in a smaller volume (50% of 1st Elution). It is also recommended to perform the 2nd elution into a separate elution tube to avoid diluting the 1st elution.

What if my incubation time varied from what is specified in the product manual?

Varying the incubation time will result in a reduction in your RNA yields.

Why do my samples show very low RNA yield?

Plasma/Serum samples contain very little cfc-RNA. This varies from individual to individual. In order to increase the yield, the amount of Plasma/Serum input could be increased.

Why does my purified cfc-RNA not perform well in downstream applications?

If a different Elution solution was used other than the one provided in the kit, the buffer should be checked for any components that may interfere with the application. Common components that are known to interfere are high salts (including EDTA), detergents and other denaturants. Check the compatibility of your Elution Buffer with the intended use.

Do I need to do a DNase treatment for my RNA Elution?

You may need to do a DNase treatment to your isolated Plasma/Serum miRNA. It is recommended to use Norgen’s RNase-Free DNase I Kit (Cat. 25710). Also please refer to the protocol for optional on column DNA removal outlined in Appendix A.

Are Norgen plasma/serum RNA purification kits compatible with blood samples from Streck Cell-Free RNA Blood Collection Tubes?

Yes, our plasma/serum RNA purification kits can be used to isolate RNA using blood samples preserved using Streck Cell-Free RNA Blood Collection Tubes.

Plasma/Serum cfc-RNA Advanced Purification Kit