Features and Benefits

Xylella fastidiosa is an Gram-negative bacteria and is transmitted by xylem fluid feeding sap insects. Diseases related with X. fastidiosa are bacterial leaf scorch, oleander leaf scorch, coffee leaf scorch (CLS), alfalfa dwarf, phony peach disease, and the economically important Pierce’s disease of grapes (PD) , citrus variegated chlorosis (CVC) and olive quick decline syndrome (OQDS) in Europe. X. fastidiosa is currently regulated in the EU as a quarantine organism. Pathogenicity of the bacterium only occurs when a large proportion of xylem vessels in a plant are colonized thus many plants are asymptomatic carriers of X. fastidiosa, which can contribute to its spread. Early and sensitive detection could prevent the spreading and help effective pest control.

For research use only and NOT intended for in vitro diagnostics.

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Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

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Documentation

Related Products

Xylella fastidiosa TaqMan Probe/Primer and Control Set

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