UltraRanger 1 kb DNA Ladder
Ideal for sizing digested DNA of high molecular weight
For research use only and NOT intended for in vitro diagnostics.
UltraRanger 1 kb DNA Ladder
Ideal for sizing digested DNA of high molecular weight
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Features and Benefits
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Fifteen discrete fragments ranging from 300 bp to 24000 bp
- Higher intensity reference bands at 5000 bp and 10000 bp
The Norgen UltraRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our UltraRanger contains fifteen discrete fragments ranging from 300 bp to 24000 bp with higher intensity reference bands at 5000 bp and 10000 bp. This ladder is ideal for sizing digested DNA of high molecular weight.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
UltraRanger 1kb DNA Ladder (Cat# 12100) - 100 loads
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
24000
|
30
|
|
2
|
14000
|
27
|
|
3
|
10000
|
60
|
|
4
|
8000
|
44
|
|
5
|
6000
|
36
|
|
6
|
5000
|
55
|
|
7
|
4000
|
49
|
|
8
|
3000
|
39
|
|
9
|
2500
|
35
|
|
10
|
2000
|
30
|
|
11
|
1500
|
33
|
| 12 | 1000 |
10 |
| 13 | 700 |
21 |
| 14 | 500 |
18 |
| 15 | 300 |
11 |
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Stable at room temperature. For longer term storage, -20°C is recommended.
Documentation
Isolation of Fractionated RNA From Hela Cells Using Norgen's Cytoplasmic Nuclear RNA Purification Kit
Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative
Determining the Molecular Weight Cut-Off of Plasma Circulating DNA Using Two Commercially Available Kits
Stability of DNA Stored in Norgen’s Saliva DNA Preservative at 55°C
Citations
| Title | The genome and proteome of Serratia bacteriophage eta which forms unstable lysogens. |
| Journal | Virology Journal. 2014. |
| Authors | Denyes JM, Krell PJ, Manderville RA, Ackermann HW, She YM, Kropinski AM. |
| Title | Red/ET recombination with chimeric oligonucleotides allows rapid generation of BAC transgenes harboring full-length or truncated huntingtin cDNA. |
| Journal | Biotechniques. 2012. |
| Authors | Hager S, L?sch S, Noll S, Khan-Vaughan L, Ehrlich M, Kranz H. |
| Title | Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. |
| Journal | Epigenomics. 2012. |
| Authors | Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y. |
| Title | Variation of Serine-Aspartate Repeats in Membrane Proteins Possibly Contributes to Staphylococcal Microevolution. |
| Journal | PLOS One. 2011. |
| Authors | Cheng J, Xue H, Zhao X. |
| Title | Unique Changes in Mitochondrial Genomes Associated with Reversions of S-Type Cytoplasmic Male Sterility in Maizemar |
| Journal | PLoS ONE. 2011. |
| Authors | Matera J, Monroe J, Smelser W, Gabay-Laughnan S, Newton K. |