Stool DNA Isolation Kit Dx
For the rapid and simple purification of bacterial and host DNA from stool and fecal samples for in vitro diagnostic use
Stool DNA Isolation Kit Dx
For the rapid and simple purification of bacterial and host DNA from stool and fecal samples for in vitro diagnostic use
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Features and Benefits
- CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
- Ideal for use in in vitro diagnostic workflows
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Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
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Eliminates PCR inhibitors including humic acids
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Fully compatible with Norgen's Stool Nucleic Acid Collection and Transport Tubes
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High quality DNA for sensitive downstream applications including PCR, qPCR, sequencing and microarray
This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx (Cat. Dx45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using Bead Tubes and a combination of chemical and physical homogenization and lysis, without the need of any phenol-chloroform extractions. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
NOTE: This product is not available for sale in the United States.
Details
Supporting Data
Kit Specifications
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Maximum Stool Input
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200 mg (fresh/frozen stool) or 400 μL (preserved stool)
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Type of Stool Processed
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Frozen, fresh or preserved stool
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Format | Spin Column |
Maximum Column Binding Capacity
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50 μg
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Maximum Column Loading Volume |
650 μL
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Elution Volume | 50 μL |
Time to Complete 10 Purifications |
30 minutes
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Applications |
PCR, qPCR, Southern Blot Analysis, |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. Dx27600 (50 preps) |
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Lysis Solution | 60 mL |
Lysis Additive | 6 mL |
Binding Solution | 7 mL |
Wash Solution I | 30 mL |
Wash Solution II | 22 mL |
Elution Buffer | 3 mL x 2 |
Bead Tube | 50 |
Mini Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
Documentation
The Isolation of High Quality Stool DNA and Its Application To Quantitative Adenovirus Detection Using Taqman Realtime PCR
Comparative Study of DNA Isolated from Stool Using Norgen’s Stool Nucleic Acid Isolation Kit and Norgen’s Stool DNA Kit
Comparison of Stool DNA Isolation Methods For Bacterial and Mammalian DNA Detection
Determination of the DNA Molecular Weight (MW) from different Norgen Columns and Isolation Methods
Gut Microbiome Diversity: Comparison of Stool DNA Preservation Methods
Citations
Title | Changes on fecal microbiota in rats exposed to permethrin during postnatal development |
Journal | Environmental Science and Pollution Research International. 2016. |
Authors | Nasuti C, Coman MM, Olek RA, Fiorini D, Verdenelli MC, Cecchini C, Silvi S, Fedeli D, Gabbianelli R |
Title | |
Journal | Biopreservation and Biobanking. 2016. |
Authors | Mathieson W, Sanchez I, Mommaerts K, Frasquilho S, Betsou F |
Title | |
Journal | Enfermedades Infecciosas y Microbiología Clínica. 2015. |
Authors | Pilar Goñi, Diego Almagro-Nievas, Joanna Cieloszyk, Silvia Lóbez, José María Navarro-Marí, José Gutiérrez-Fernández |
Title | |
Journal | Western Pacific Surveillance and Response Journal. 2014. |
Authors | Aruna Devi, Jenny Wilkinson, Timothy Mahony and Thiru Vanniasinkam |
Title | How to make DNA count: DNA-based diagnostic tools in veterinary parasitology. |
Journal | Veterinary Parasitology. 2011. |
Authors | Hunt PW, Lello J. |