Plum Pox Virus (PPV) TaqMan RT-PCR Detection Kits
For research use only and NOT intended for in vitro diagnostics.
Plum Pox Virus (PPV) TaqMan RT-PCR Detection Kits
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Features and Benefits
- Detection kits for PPV
- Available in TaqMan format for analysis
Plum pox virus (PPV) is a member of the genus Potyvirus, and the virus is considered the most serious pathogen affecting stone fruits including apricots, cherry, nectarine, peaches, and plums. The symptoms of plum pox disease or Sharka include fruit abnormality, loss of taste, and fruits that drop prematurely. These symptoms may cause losses as high as 80–100% of a crop and consequently it is a disease of economic significance in every country where stone fruits are grown. To achieve successful eradication or effective management of the associated disease, sensitive detection of the virus in infected plants is required.
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- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Details
Supporting Data
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM33450 (100 preps) | Cat. TM33410 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | - |
PPV Primer & Probe Mix | 280 μL | 280 μL |
PPV Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |