Features and Benefits

Phomopsis cane and leaf spot, caused by Phomopsis viticola, occurs in most grape-growing regions and is an important disease of grapes worldwide. The fungus attacks all green parts of the vine, including canes, leaves, flowers, rachises, and berries. However, most economic damage is caused by rachis and berry infection. Infected leaves have small, yellowish spots with dark brown centers and may be puckered. With successive cool, wet springs, the inoculum will build up and infections will become more severe over time. Since symptoms appear 21 to 30 days after infection, early detection of the pathogen in the vineyard is therefore crucial for its control.

For research use only and NOT intended for in vitro diagnostics.

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Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

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