Name: microRNA (cel-miR-39) Spike-In Kit
Brand: Norgen Biotek Corp.
SKU: 59000
Price: 55.000000 USD
Availability: InStock

Supporting Data

Figure 1. Accurate Quantity and High Quality cel-miR-39 Spike-In. Various quantities of the reconstituted cel-miR-39 RNA was used as spike-in during isolation of urinary RNA (1 mL) using Norgen's Urine Exosome RNA Isolation Kit (Cat. 47200). Isolated RNA was reverse transcribed using Norgen's microScript cDNA Synthesis Kit (Cat. 54410), followed by qPCR using Norgen's 2X PCR Master Mix (Cat. 28007), supplemented with SYBR Green I. The cel-miR-39 RNA was successfully detected in all samples, with the Ct values distributed according to the amount of RNA spiked-in.

Figure 2. Successful Incorporation of cel-miR-39 Spike-In into Small RNA-Seq Library. Small RNA-Seq Library was generated using either a competitor’s or Norgen's cel-miR-39 Spike-In RNA at two concentrations (High and Low). The library was generated using Illumina's TruSeq Small RNA Library Preparation Kit. The incorporated libraries were resolved on a PAGE gel. Only Norgen's cel-miR-39 RNA showed effective incorporation into small RNA-Seq library (Red Arrows). The competitor's cel-miR-39 RNA only yielded bands (Yellow Arrows) equivalent to the No Template Control (NTC) that are adapter dimers.

Storage Conditions
Upon receipt, store Norgen’s microRNA (cel-miR-39) Spike-In Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.

Component	Cat. 59000
cel-miR-39 RNA	10 pmol
cel-miR-39 Forward PCR Primer	1 nmol
Nuclease-Free Water	1.25 mL
Product Insert	1

microRNA (cel-miR-39) Spike-In Kit