Features and Benefits

Mastitis is an infection in the mammary gland that causes inflammation in the udder or breast tissues. There is a wide range of bacterial pathogens that can cause mastitis, some of which include Brucella abortus, Listeria monocytogenes, and Staphylococcus aureus.

Multiplexed TaqMan PCR-based kit for Mastitis Pathogens detection. Norgen's turn-key solution comes with Master Mix, Primer & Probe Mix, Positive control, and Nuclease free water. Norgen's TaqMan Master Mix includes an internal PCR control to monitor PCR inhibition and validate sample quality.

Details

Supporting Data

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Figure 1.  Example of TaqMan One-step RT-PCR Positive result. PCR signals above the baseline from FAM, Tex615, Cy5, and HEX channel indicate the successful PCR. Individual amplification in any of the channels also indicates a positive result for the pathogen corresponding to that channel.

Figure 2.  Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM,TEX615, and Cy5 channels but amplification signal from HEX indicates the successful PCR.

Figure 3.  Example of TaqMan one-step RT-PCR inhibition result. No signal from all targets was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

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