Features and Benefits

Norgen’s Low Abundance DNA Quantification Kit offers a PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range. The kit consists of a specially designed primer mix, that is used in conjunction with the provided 2x PCR Master Mix, to amplify human DNA from different types of inputs (such as various liquid biopsies). The kit contains a fluorescent DNA dye, and is compatible with any Real-Time PCR system with FAm/SYBR Green detection. The unknown DNA is accurately quantified by using a standard curve constructed from the provided DNA Standard.

Details

Supporting Data

Previous

Figure 1. Sensitivity of DNA Quantification of the Low Abundance DNA Quantification Kit Compared to Other Methods. A diagram representing the dynamic range of different DNA quantification methods is presented here. The Low Abundance DNA Quantification Kit can quantify purified DNA from low abundance samples in the pg and sub-pg range.

Figure 2. Sensitivity of DNA Quantification in the Picogram Range Using the Low Abundance DNA Quantification Kit. A representative qPCR Baseline Graph showing the amplification of a DNA standard dilution series. The Low Abundance DNA Quantification Kit can quantify purified DNA from low abundance samples such as liquid biopsies (Plasma or Urine). As little as 500 fg of DNA can be quantified.

1. 
2. 

Next

Click for expanded view

Documentation

Related Products

Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit

Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit

Plasma/Serum RNA Purification Maxi Kit