Features and Benefits

The Hop Stunt Viroid (HSVd) is one of a group of the smallest self-replicating RNAs pathogenic to plants. The viroid is a covalently closed, circular, single-stranded RNA consisting of 297 nucleotides. Strains of HSVd can be found to infect citrus, grapes, stone fruit and cucumber, and have been detected in Europe, Asia, North America, Australia and the Middle East. To prevent or reduce the negative effects caused by the viroid it is necessary to use viroid-free planting material. Methods for the identification of this viroid in host plants should help to control the spread of this agriculturally important plant pathogen.

For research use only and NOT intended for in vitro diagnostics.

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Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

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Documentation

Related Products

Hop Stunt Viroid (HSVd) TaqMan Probe/Primer and Control Set

Plant/Fungi Total RNA Purification Kit