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E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.

For research use only and NOT intended for in vitro diagnostics.

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Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals (FAM and Cy5) above the baseline from FAM, Cy5 and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Positive (Cy5) result. Both PCR signals above the baseline from Cy5 and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan PCR Positive (FAM) result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 4. Example of TaqMan PCR Negative result. No target DNA was detected in FAM and Cy5 channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 5. Example of TaqMan PCR inhibition result. No signal from FAM, Cy5 and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

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