COVID-19/Influenza (A & B) TaqMan RT-PCR Detection Kit
For research use only and NOT intended for in vitro diagnostics.
Detect SARS-CoV-2 and Influenza A + B in one convenient kit
COVID-19/Influenza (A & B) TaqMan RT-PCR Detection Kit
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Features and Benefits
- Multiplex RT-PCR system detects SARS-CoV-2 specific gene (N gene), Influenza A (M gene) and Influenza B (HA gene) in a single one step RT-PCR reaction
- Primer & Probe Mixes contain all N gene, M gene, HA gene and human RP assays
- Positive Control contains the SARS-related target genes: N gene, Influenza A target gene (M gene), Influenza B target gene (HA gene) as well as the human RP (internal control)
- Convenient ready-to-use 2X One-Step RT-PCR Master Mix included in kit
- Manufactured under ISO 13485:2016 for research use only
- CE-IVD marked version pending
Norgen’s COVID-19/Influenza (A & B) TaqMan RT-PCR Kit includes 2X One-Step RT-PCR Master Mix, a primer/probe mix, a positive control and a negative control (nuclease-free water). The primer/probe mix contains SARS-CoV-2 detection target; N (Nucleoprotein), Influenza A detection target; M gene and Influenza B detection target; HA (Hemaglutinin) and the human RNase P transcript as an internal control target to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The provided N/Inf A & B/RP Positive Control contains an in vitro RNA transcript for the SARS-related target gene (N Gene), and genes associated with Influenza A (M gene) and Influenza B (HA Gene), as well as the human RP gene (internal control).
Norgen’s COVID-19/Influenza (A & B) TaqMan RT-PCR Kit was developed and validated to be used with the following PCR instruments:
- Qiagen Rotor-Gene Q, BioRad CFX96 Touch™ Real-Time PCR Detection System, ABI 7500, ABI Quantstudio 5 and 7.
Details
Supporting Data
Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 6 months at -20°C without showing any reduction in performance.
Kit Component | Cat. TM67400 (500 preps) |
---|---|
N/Inf A & B/RP Primer & Probe Mix | 850 μL |
N/Inf A & B/RP Positive Control † | 500 μL |
2X One-Step RT-PCR Master Mix | 9 x 1 mL |
Nuclease-Free Water (Negative Control) | 2 x 1.25 mL |
Product Insert | 1 |
† Contains an in vitro RNA transcript for the SARS-CoV-2 N gene, Influenza M and HA genes and RNase P RNA fragments.