Chrysanthemum chlorotic mottle viroid (CChMVd) TaqMan RT-PCR Detection Kits

Features and Benefits

Detection kits for the CChMVd
Available in TaqMan format for analysis

Viroids are the smallest known agents causing infectious diseases. The pathogens are small (246–401 nucleotides), circular, single-stranded RNAs that do not code for any protein. Chrysanthemum chlorotic mottle viroid (CChMVd) is one of two viroids that infect chrysanthemum (Dendranthema grandiflorum). CChMVd infections of many chrysanthemum cultivars have been a severe problem in the world.

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CChMVd TaqMan RT-PCR Kit, 100 reactions

Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents

CChMVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM35550 (100 preps)	Cat. TM35510 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	-
CChMVd Primer & Probe Mix	280 μL	280 μL
CChMVd Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Chrysanthemum chlorotic mottle viroid (CChMVd) TaqMan RT-PCR Detection Kits