50 bp DNA Ladder
Ideal for PCR product size determination
For research use only and NOT intended for in vitro diagnostics.
50 bp DNA Ladder
Ideal for PCR product size determination
$75.00
SKU
13525
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Features and Benefits
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- 10 discrete bands from 50 bp to 500 bp
- Higher intensity reference band at 250 bp
The 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at 250 bp. This Ladder is ideal for quick sizing of PCR products
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
50 bp DNA Ladder (Cat# 13525) - 100 loads
Ladder Properties:
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
500
|
79
|
|
2
|
450
|
67
|
|
3
|
400 |
59
|
|
4
|
350 |
55
|
|
5
|
300 |
49
|
|
6
|
250 |
76
|
|
7
|
200 |
33
|
|
8
|
150 |
22
|
|
9
|
100
|
31
|
|
10
|
50 |
29
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
Citations
| Title | Fluorescent carbon nanoparticle-based lateral flow biosensor for ultrasensitive detection of DNA |
| Citation | Biosensors and Bioelectronics 2023. |
| Authors | Takalkar, S., Baryeh, K., & Liu, G. (2017). |
| Title | Inter-simple Sequence Repeat (ISSR) Analysis of Somaclonal Variation in Artemisia amygdalina Decne, plantlets Regenerated from nodal explants |
| Citation | Journal on New Biological Reports 2023. |
| Authors | M. Khan, B.A. Ganai, A.N. Kamili, M. Akbar |
| Title | Microsatellite instability at three microsatellite loci (D6mit3, D9mit2 and D15Mgh1) located in different common fragile sites of rats exposed to cadmium |
| Citation | Mutation Research 696 (2010) 160-166 2023. |
| Authors | Akmal A. El-Ghora, Magda M. Noshya, Hassan M. El Ashmaouib, Jehane I. Eida,*, Mohamed S. Hassananeb |
| Title | Two Novel Variants Affecting CDKL5 Transcript Associated with Epileptic Encephalopathy |
| Citation | Genetic Testing and Molecular Biomarkers 2023. |
| Authors | Neupauerová, J., Šterbová, K., Vlcková, M., Sebronová, V., Maríková, T. Á., Krutová, M., ... & Laššuthová, P. (2017). |