Name: Saliva DNA Collection and Preservation Devices
Brand: Norgen Biotek Corp.
SKU: 49000
Price: 715.000000 USD
Availability: InStock

Features and Benefits

CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Ideal for use in in vitro diagnostic workflows
Reliable and cost-effective
Non-invasive, user-friendly sample collection
Sample collection and preservation in one convenient kit
Samples are non-infectious and can be handled and shipped safely
Preserved DNA is stable for 2 years at ambient temperature
High quality DNA is suitable for sensitive downstream applications including PCR, qPCR, sequencing, SNP analysis, microarrays, RFLP and Southern Blot Analysis
Shipping accessories can be purchased separately

Norgen's Saliva DNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of DNA in saliva samples at ambient temperature. Each of the 50 Saliva DNA Collection and Preservation Devices consists of 3 components: (1) Saliva Collection Funnel and Collection Tube, (2) Collection Tube Cap, and (3) Norgen's Saliva DNA Preservative contained within a sealed squeezable ampoule. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. DNA can be isolated from the preserved saliva samples using Norgen's Saliva DNA Isolation Kit (Cat# Dx45400). Each of Norgen's Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 2 years at room temperature. This kit is ideal for collecting and preserving DNA samples for in vitro diagnostic use for medical purposes.

Saliva DNA Preservative

Norgen's Saliva DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The buffer prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for more than 2 years without any detectable DNA degradation.

DNA Isolation from Preservative

Prior to saliva DNA isolation, vortex the Collection Tube containing preserved saliva for 10 seconds and incubate at 55°C for one hour. Saliva DNA can now be isolated from the preserved saliva samples using Norgen’s spin-column based Saliva DNA Isolation Kit Dx (Cat# Dx45400) or other commercially available methods that have been validated for use with Norgen’s Saliva DNA Collection and Preservation Devices.

NOTE: This product is not available for sale in the United States.

Details

Supporting Data

Figure 1. Stability of DNA Preserved in Norgen's Saliva DNA Preservative at Room Temperature for over 2 Years. Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Saliva DNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 24 months. Saliva DNA was subsequently isolated at 4 months, 8 months, 16 months and 24 months from 0.5 mL of the saliva/preservative sample using Norgen's Saliva DNA Isolation Kit (Cat. RU45400). For visual analysis, 100 µL of the purified DNA was run on an agarose TAE gel. As it can be seen, there is no evidence of DNA degradation after the saliva samples are stored for 24 months at ambient temperatures in Norgen's Saliva DNA Preservative. Furthermore, the size of the DNA was maintained over 24 kb for the entire 24 month period. M: Norgen's UltraRanger 1 Kb DNA Ladder (Cat. 12100).

Figure 2. GAPDH Real Time PCR Amplification of Saliva DNA from Saliva Samples Stored at Room Temperature from Day 0 up to 24 Months. All isolated saliva DNA samples were successfully amplified without any sign of PCR inhibition and difference in Ct. Red: Day 0, Blue: 4 months, Purple: 8 months, Green: 16 months and Sky blue: 24 months. Black: No template control.

Figure 3. V3-V4 16s rRNA PCR amplification for Illumina MiSeq 16s rRNA library preparation. The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 2 years at room temperature in Norgen’s Saliva DNA Preservative. The PCR result shows the clean PCR amplification at ~550 bp indicating the right target of V3-V4 16s rRNA , with no difference between day 0 and 24 months. M: FastRunner DNA Ladder (Cat. 12800)

Kit Specifications
Volume of Saliva Collected	2 mL
Volume of Saliva-Preservative Mix	4 mL
Preservation Temperature	Room Temperature
Preservation time	Over 2 years at room temperature

Shelf Life and Handling

The Collection Device should be kept tightly sealed and stored at room temperature for up to 3 years without any reduction in kit performance (The collection due date is written on the device label).
Once collected, saliva is stable for more than 2 years when kept tightly sealed and stored at room temperature.
The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.

Kit Components	Cat. 49000 (50 Devices)
Individual Saliva DNA Collection and Preservation Devices	50
Donor Procedure Flowchart	1
Product Insert	1

Individual Saliva DNA Collection and Preservation Device Contents
Saliva Collection Funnel and Collection Tube	1
Collection Tube Cap	1
Preservative Ampoule	1
Donor Instructions	1

Documentation

PROTOCOLS

(49000) Saliva DNA Collection and Preservation Devices - Protocol (50 devices)

APPLICATION NOTES

High Efficiency Real-Time PCR from Saliva DNA

Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative

The Range of DNA Yield with Norgen’s Saliva DNA Collection, Preservation and Isolation Kit

Sequencing Analysis of Saliva DNA Isolated using Norgen’s Saliva DNA Collection, Preservation and Isolation Kit

Isolation of High Quality Saliva DNA from Variable Donor Saliva Samples

Inhibitory Effect of Norgen’s Saliva DNA Preservative on the Growth of Bacteria and Yeast

A Comparative Study Between Two Saliva DNA Preservation Systems, and Two Column-Based Saliva DNA Purification Methods

Efficient Restriction Enzyme Digestion of Saliva DNA isolated using Norgen's Saliva DNA Collection, Preservation and Isolation Kits

Comparison of Norgen’s Saliva DNA Collection, Preservation and Isolation Kit to a Leading Competitor’s Kit

DNA Isolation from Saliva Preserved using Norgen’s Saliva DNA Collection and Preservation Device using Beckman Coulter Agencourt

DNA Isolation from Saliva Preserved with Norgen’s Saliva DNA Collection and Preservation Device using Qiagen’s QIAamp DNA Blood

Stability of DNA Stored in Norgen’s Saliva DNA Preservative for 52 Months at Room Temperature

Sex Determination from Saliva DNA Isolated using Norgen's Saliva DNA Collection, Preservation, and Isolation Kit

The Range of DNA Yield and Purity with Norgen's Saliva DNA Collection, Preservation, and Isolation Kit

Column-Based Isolation of DNA from Preserved Saliva Samples Offers High Quality, Consistent, and Rapid DNA Isolation

Citations

Title	CYP3A genetic variation and taxane-induced peripheral neuropathy: a systematic review, meta-analysis, and candidate gene study
Citation	Frontiers in Pharmacology 2023.
Authors	Laurence McEvoy, Joanne Cliff, Daniel F Carr, Andrea Jorgensen, Rosemary Lord, and Munir Pirmohamed

Title	Three siblings with Prader-Willi syndrome caused by imprinting center microdeletions and review
Citation	American Journal of Medical Genetics 2018.
Authors	Hartin, S. N., Hossain, W. A., Weisensel, N., & Butler, M. G. (2018)

Title	Detection of Novel Integrons in the Metagenome of Human Saliva
Citation	PLoS One 2016.
Authors	Tansirichaiya, S., Rahman, M. A., Antepowicz, A., Mullany, P., & Roberts, A. P

Title	Increased risk of attention-deficit/hyperactivity disorder associated with exposure to organophosphate pesticide in Taiwanese children
Citation	Andrology 2016.
Authors	Yu, C. J., Du, J. C., Chiou, H. C., Chung, M. Y., Yang, W., Chen, Y. S., ... & Chen, M. L.

Title	Sugar-Sweetened Beverage Consumption Is Adversely Associated with Childhood Attention Deficit/Hyperactivity Disorder
Citation	International Journal of Environmental Research and Public Health 2016.
Authors	Yu, C. J., Du, J. C., Chiou, H. C., Feng, C. C., Chung, M. Y., Yang, W., ... & Chen, M. L

Title	Association of prementrual/menstrual symptoms with perinatal depression and a polymorphic repear in the polyglutamine tract of the retinoic acid induced 1 gene
Citation	Journal of Affective Disorders 2014.
Authors	E Tan, H Tan, T Chua, T Lee, J Ng, Y Ch'ng, C Choo, HY Chen

Title	RANL/RANKL/OPG Pathway: Genetic associations with Stress Fracture period prevalence in Elite Athletes
Citation	Bone 2014.
Authors	I Varley, DC Hughes, JP Greeves, T Stellingwerff, C Ranson, WD Fraser, C Sale

Saliva DNA Collection and Preservation Devices Dx