Features and Benefits

• Eight discrete bands, ranging from 100 to 1,000 bases
• Higher intensity band at 500 bases for easy reference
• No need for staining or destaining as loading dye contains ethidium bromide
• Convenient lyophilized format provides better product stability

The Norgen 100 b RNA Ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of small RNA molecules using native or denaturing agarose gels, and can be easily visualized under UV.

Contents

• 2 vials of lyophilized RNA ladder (25 loads each)
• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
• 2x loading buffer

Details

Supporting Data

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 Figure 1: 100 b RNA Ladder 

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Specifications:

• Eight discrete bands, ranging from 100 to 1,000 bases
• Higher intensity band at 500 bases for easy reference

Contents:

• 2 vials of lyophilized RNA ladder (25 loads each)
• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
• 2x loading buffer

Instructions:

 

To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently.
Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1.5-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. There is no need for staining and destaining denaturing gels since Norgen’s loading buffer contains ethidium bromide.

 

Storage:

 

Store at -20°C.

Documentation

PROTOCOLS

15002 - Product Insert

SAFETY DATA SHEETS

15002 - 100 B RNA Ladder - SDS

Citations

Title	Duplex reverse transcription-polymerase chain reaction system to detect Potato spindle tuber viroid using an internal control mRNA and a non-infectious positive control RNA
Journal	Journal of General Plant Pathology. 2009.
Authors	Hataya T.