Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15–25°C). This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Adding more or less than the specified volumes may reduce both the quality and quantity of the purified RNA. Eluting your RNA in high volumes will increase the yield but will lower the concentration. Eluting in small volumes will increase the concentration but will lower the overall yield.
Your purified RNA will be contaminated with the Wash Solution A. This may reduce the quality of your purified RNA and will interfere with your downstream applications.
Exosomes contain very little RNA. This varies from individual to individual based on numerous variables. In order to increase the yield, the amount of urine, plasma or serum input could be increased.
Most of the Exosomal RNA is short RNA fragments with a very low concentration, where the A260/280 ratio tends to decrease with the decrease in the RNA concentration. The A260/280 ratio is normally between 1 – 1.6. This low A260/280 ratio will not affect any downstream application.
If a different Elution Buffer was used other than the one provided in the kit, the buffer should be checked for any components that may interfere with the application. Common components that are known to interfere are high salts (including EDTA), detergents and other denaturants. Check the compatibility of your elution buffer with the intended use.
