EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits

Supporting Data

Figure 1. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Mini Kit. Calculations were relative to raw reads.

Figure 2. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Mini Kit.

Figure 3. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Mini Kit.

Figure 4. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Midi Kit. Calculations were relative to raw reads.

Figure 5. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Midi Kit.

Figure 6. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Midi Kit.

Figure 7. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit. Calculations were relative to raw reads.

Figure 8. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit.

Figure 9. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit.

Sample Type	Cell-Free Cell Culture Media
Sample Volume Range	5 ml to 10 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Documentation

EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits