ProteoSpin™ On-Column Proteolytic Digestion Kit
For research use only and NOT intended for in vitro diagnostics.
ProteoSpin™ On-Column Proteolytic Digestion Kit
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Supporting Data
Figure 1. Generation of Tryptic BSA Peptides without the Generation of any Artifacts. MALDI-TOF spectra of the peptides that resulted after BSA was digested on-column with trypsin using the ProteoSpinTM On-Column Proteolytic Digestion Kit (Panel A) or digested in a typical liquid environment (Panel B). For both digestions, 15 µg of BSA was used as the input and 0.3 µg of Trypsin was used for the digestion. For on-column digestion, the 15 µg of BSA was loaded onto the column with the trypsin, the BSA was washed, then the on-column digestion proceeded and the resulting peptides were eluted. By comparing the spectra, it can be seen that the ProteoSpinTM kit (Panel A) resulted in the generation of the same peptide profile in MALDI-TOF as the liquid digestion did (Panel B). Furthermore, no artifacts were found to arise from the use of the column, and all the same peaks could be detected in both spectra.
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Kit Specifications
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| Maximum Protein Input |
15 μg
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| Minimum Protein Input |
2 μg
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| Minimum Elution Volume |
30 μL
|
| Time to Process 10 Purifications |
20 minutes
(Plus a 1-hour incubation) |
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 17500 (25 preps) |
|---|---|
| Wash Solution C | 30 mL |
| Binding Buffer A | 4 mL |
| Column Activation Buffer | 3 mL |
| Micro Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution tubes (1.7 mL) | 25 |
| Product Insert | 1 |