TruScript Reverse Transcriptase and Kits

Supporting Data

Figure 1. Robust Reverse Transcription of All RNA Transcripts. Norgen's TruScript Reverse Transcriptase is a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase that can produce cDNA from different kinds of RNA transcripts, including those with high difficulty or a high degree of secondary structure. In Panel A, TruScript Reverse Transcriptase was compared with a competitor's M-MuLV Reverse Transcriptase for RT-PCR of two different RNA transcripts. Norgen's TruScript showed good amplification for both targets while the competitor failed to amplify Target #2 with good quantity. In Panel B, TruScript Reverse Transcriptase was compared with a competitor's M-MuLV Reverse Transcriptase in an RT-qPCR reaction. Norgen's TruScript Reverse Transcriptase provided a better amplification as shown with a lower Ct value.

Figure 2. High Sensitivity with Robust Amplification from Different Amounts of RNA Input. Norgen’s Norgen’s TruScript Reverse Transcriptase was used in an RT-qPCR reaction to amplify the human 5S rRNA transcripts from a dilution series of HeLa total RNA isolated using Norgen’s Total RNA Purification Kit. The PCR target was robustly amplified from a broad range of RNA inputs, from the 10 ng down to 1 pg.

TruScript First Strand cDNA Synthesis Kit (Cat.# 54420) - Kit Components

1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert

Storage Conditions and Product Stability

Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. 54440 (10,000 units)	Cat. 54420 (50 rxns)	Cat. 54410 (50 rxns)
TruScript™ Reverse Transcriptase (200 units/ μL)	50 μL	-	-
5x RT Buffer	1 mL	-	-
TruScript™ Enzyme Mix	-	100 μL	100 μL
2x Reaction Mix	-	500 μL	-
2x Reaction Mix for mRNA	-	-	500 μL
Nuclease-Free Water	1.25 mL	1.25 mL	1.25 mL
Product Insert	1	1	1

Documentation

PROTOCOLS

54420 - TruScript First Strand cDNA Synthesis Kit Protocol (50 rxns)

54440 - TruScript Reverse Transcriptase Protocol (10,000 units)

54400 - TruScript First Strand cDNA Synthesis Kit for mRNA Protocol (50 rxns)

TruScript Reverse Transcriptase and Kits