ITS2 Library Preparation Kit for Illumina
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For research use only and NOT intended for in vitro diagnostics.
ITS2 Library Preparation Kit for Illumina
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Supporting Data
Figure 1. Amplicons generated by ITS2 amplicon primers, cleaned and run on a 2% agarose gel. The figure represents technical replicates of the standard DNA and No template control.
Figure 2. Libraries generated by indexing PCR, cleaned and run on a 2% agarose gel. The figure represents technical replicates of the standard DNA.
Figure 3. Bioanalyzer traces of ITS2 libraries generated by indexing PCR, cleaned and run on a high sensitivity DNA chip. The figure represents the library generated using standard fungal DNA.
Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s ITS2 Library Prep Kit for Illumina is shipped as one kit box (for
the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be
stored at -20°C upon arrival.
All kit components will remain stable for at least 1 year when stored at the specified storage conditions.
Step | Component | Cat. 71100 (24 preps) | Cat. 71110, 71120, 71130, 71140 (96 preps) | Tube Cap |
---|---|---|---|---|
Amplicon PCR (PCR 1) | MGX Master Mix | 330 μL | 1320 μL | |
ITS2 Primer Mix | 70 mL | 280 μL | ||
Index PCR (PCR 2) | Indexing Master Mix | 660 μL | 2 x 1320 μL | |
N7xx Index Primer | 50 μL | 50 μL | ||
S5xx Index Primer | 70 μL | 70 μL | ||
PCR Clean-Up | Resuspension Buffer | 2 x 1250 μL | 2 x 5000 μL | |
Nuclease-free Water | 1250 μL | 1 x 6000 μL | White |