Figure 2. The analytical specificity of Norgen’s COVID-19 TaqMan RT-PCR Kit (E/RdRP gene)(Cat. TM67200) was evaluated by testing a panel of RNA from related pathogens. Oropharyngeal swabs were collected from healthy donors and were spiked with RNA from each pathogen. Total RNA was then purified using Norgen's Total RNA Purification Kit (Cat. 17200). A one-step RT-PCR for detection of the four targets was performed using Norgen’s COVID-19 TaqMan RT-PCR Kit (E/RdRP gene) (Cat. TM67200). Norgen’s COVID-19 TaqMan RT-PCR Kit (E/RdRP gene) (Cat. TM67200) did not cross-react with any of the specified organisms. 

Figure 3. Clinical testing of the COVID-19 TaqMan RT-PCR Kit (E/RdRP gene)(Cat. TM67200) was conducted with contrived oropharyngeal swabs (circle) and nasopharyngeal swabs (cross).  Swab samples were collected from healthy donors and placed into preservative using Norgen’s Total Nucleic Acid Preservation Tubes (Cat. 69200).  To prepare the contrived positive samples, the preserved swab samples were spiked with 100,000 copies of positive control. Total viral RNA was then isolated using Norgen’s Total RNA Purification Kit (Cat. 17200).  Following RNA isolation, one-step RT-PCR for detection of the four targets was performed using Norgen’s COVID-19 TaqMan RT-PCR Kit (E/RdRP gene) (TM67200).