RNA Preserve

Supporting Data

Figure 1. Soil RNA Preservation up to 2 weeks at Room Temperature using Norgen's RNA Preserve. Freshly collected (200 mg of clay) was mixed with 400 µL of three different RNA preservatives (Norgen’s RNA Preserve, Competitor A and Competitor B). Samples were then stored at room temperature for 2 weeks. Prior to RNA purification, the RNA preservative was removed by a short centrifugation step (5,000 rpm for 3 minutes). Next, RNA was purified from each sample using Norgen’s Soil Total RNA Purification Kit (Cat. 27750). Purified total soil RNA was visualized on 1x MOPS RNA gel. Result indicated that Norgen's RNA Preserve could maintain the integrity of Soil RNA for 2 weeks of storage at room temperature.

Figure 2. Plant RNA Stability after 2 Days Incubation at Room Temperature using Norgen’s RNA Preserve. Six leaf disks (0.7 cm diameter) were stored at different storage conditions (in Norgen’s RNA Preserve, not preserved and stored at room temperature, -70°C) for 2 days and total RNA was then purified using Norgen’s Plant/Fungi Total RNA Purification Kit (Cat. 25800). The purified RNA was loaded onto a Nano RNA chip and analyzed in 2100 Expert (v. B.02.08.SI648, Agilent Technology). The RNA preserved in Norgen's RNA Preserve shows a similar RNA profile with the frozen method, indicating no changes in RNA stability at room temperature for 2 days with Norgen’s RNA Preserve, while the not preserved samples start showing signs of degradation after 2 days (red box and arrow).

Storage and Stability
Norgen’s RNA Preserve can be stored at room temperature for up to 6 months. If precipitate is observed, warm at 37ºC for 30 minutes.