Supporting Data

Previous

Figure 1:  MidRanger 1kb DNA Ladder

1. 

Next

Click for expanded view

MidRanger 1kb DNA Ladder (Cat# 11700) - 100 loads

Ladder Properties:
• Ten discrete fragments ranging from 300 bp to 5000 bp

 

Fragment	Size (bp)	Mass (ng)
1	5000	88
2	4000	78
3	3000	67
4	2500	58
5	2000	50
6	1500	43
7	1000	25
8	700	34
9	500	33
10	300	24

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

 

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Documentation

PROTOCOLS

11700 - Product Insert

APPLICATION NOTES

DNA Quantification by Gel Densitometry with Norgen DNA Ladders

SAFETY DATA SHEETS

11700 - MidRanger 1 KB DNA Ladder - SDS