Saliva Exosome Purification Kit
For research use only and NOT intended for in vitro diagnostics.
Saliva Exosome Purification Kit
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Kit Specifications
|
|
Saliva Input
|
500μL - 2mL
|
Size of Exosome Purified
|
40nm - 150nm
|
Elution Volume
|
200μL
|
Time to Complete 10 Purifications |
15 - 30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat No. 65300 (50 preps) |
---|---|
Slurry E | 12.5 mL |
ExoC Buffer | 1.5 mL |
ExoR Buffer | 12 mL |
Mini Filter Spin Columns inserted into 2 mL tubes | 50 |
Product Insert | 1 |
Documentation
FAQs
Spin Column
Simply remix and recentrifuge. After centrifuging decant the supernatant
Adding less volume may reduce the amount of the purified exosomes. Adding more may not affect the exosome capture but may affect the release of the purified exosomes in the ExoR Buffer.
Adding a different volume from the specified optimum volume will significantly reduce the amount of the purified exosomes.
Adding less volume will reduce the release of the captured exosomes in the ExoR Buffer. Adding more will not affect the release of the captured exosomes but it will be more diluted.
Any grey resin will be filtered through the Mini Filter Spin Column and the flowthrough which contains the purified exosomes should not contain any grey resin.
ExoR buffer is compatible with most proteomic downstream applications. The ExoR buffer doesn't contain any detergents. Please contact our Tech support team at support@norgenbiotek.com and ask for reference publications.
If not using for downstream application immediately, it is recommended to store the purified exosomes at -80 degrees.
Yes, the exosomes purified using Norgen Biotek's technology are biology active. Please contact our Tech support team at support@norgenbiotek.com and ask for reference publications.