Supporting Data

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Figure 1:  LowRanger 100 bp DNA Ladder

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LowRanger 100bp DNA Ladder (Cat# 11500) - 100 loads

Ladder Properties:
• Eleven discrete bands, ranging from 100 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference.

 

Fragment	Size (bp)	Mass (ng)
1	2000	110
2	1500	83
3	1000	55
4	800	44
5	700	39
6	600	33
7	500	55
8	400	22
9	300	17
10	200	22
11	100	22

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

 

Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Documentation

PROTOCOLS

11500 - Product Insert

APPLICATION NOTES

DNA Quantification by Gel Densitometry with Norgen DNA Ladders

SAFETY DATA SHEETS

11500 - LowRanger 100 BP DNA Ladder - SDS