Figure 1. Higher Yields of DNA than Competitor Z. Stool DNA was isolated from 200 mg of fresh stool samples using Norgen’s Stool DNA Isolation Kit Dx and Competitor Z's Kit. For evaluation, 10 µL of DNA from the elution was run on 1X TAE 1.2% agarose gel. Norgen's kit isolated much higher yields of DNA. Marker= Norgen’s HighRanger DNA Ladder.
Figure 2. Higher Quality DNA than Competitor Z. Stool DNA was isolated from 200 mg of fresh stool samples using Norgen's Stool DNA Isolation Kit Dx and Competitor Z's Kit. DNA quality was then was measured by Nanodrop. Norgen's Stool DNA Isolation Kit Dx isolated DNA of the highest quality.
Figure 3. High Quality DNA confirmed by Real-time PCR. Stool DNA was isolated from 200 mg of fresh stool samples using Norgen's Stool DNA Isolation Kit Dx and Competitor Z's Kit. DNA quality was confirmed by real-time PCR using 4 µL of stool DNA (total PCR reaction volume was 20 µL) to detect 16S rRNA from different stool samples. The earlier Ct value with Norgen's DNA samples (blue lines) compared to Competitor Z's samples (red lines) indicated a higher quality of stool DNA for downstream applications.
Figure 4. Taxonomic Classifications of 10 Fecal Microbiomes. A) Principal Coordinate Analysis of 10 fecal microbiomes showing differences in the distribution of taxonomic classifications between samples up to class level. B) Hierarchical clustering of 10 fecal microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.
200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed
Frozen, fresh or preserved stool
Format
Spin Column
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Elution Volume
50 μL
Time to Complete 10 Purifications
30 minutes
Applications
PCR, qPCR, Southern Blot Analysis,
Sequencing, Microarray Analysis.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
