Biofilm DNA Isolation Kit
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For research use only and NOT intended for in vitro diagnostics.
Biofilm DNA Isolation Kit
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Supporting Data
Figure 1. High quality and yield of DNA from biofilm formed by two different species, Komagataeibacter xylinus (1-4), Komagataeibacter hansenii (5-8). Total DNA was isolated from 200 mg of biofilm using Norgen's Biofilm DNA Isolation Kit. For evaluation, 10 µL of each 100 µL DNA elution was run on a 1.2 % agarose gel. Note the high yield and quality of the DNA in all lanes. Lane M: Norgen's HighRanger 1 kb DNA Ladder (cat. 11900).
Figure 2. High DNA quality. DNA was isolated from a number of bioflim using Norgen's Biofilm DNA Isolation Kit. All DNA elutions isolated using Norgen’s Biofilm DNA Isolation Kit showed an excellent DNA quality indicating the consistent and robust performance of the Biofilm DNA Isolation Kit.
Figure 3. Amplification of bacterial cellulose synthesis (bcs), a gene from Komagataeibacter xylinus (circle) and Komagataeibacter hansenii (cross). From 100 µL elution, 5 µL of biofilm DNA was directly mixed to real-time PCR master mix (total reaction volume 20 µL), and the real-time SYBR Green PCR reaction was performed. The bcs gene was successfully amplified from the different samples without any inhibition and at the same Ct, indicating the excellent quality of biofilm DNA for downstream applications.
Kit Specifications
|
|
Maximum Column Binding Capacity |
50 µg
|
Maximum Column Loading Volume |
650 µL
|
Minimum Biofilm Input (wet weight) | 50 mg |
Maximum Biofilm Input (wet weight) |
200 mg
|
Average Yield from 200 mg of biofilm* |
2-4 µg
|
Time to Complete 10 Purifications | 45 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 62300 (50 preps) |
---|---|
Lysis Buffer L | 30 mL |
Lysis Additive A | 6 mL |
Binding Buffer I | 7 mL |
Wash Solution A | 18 mL |
Elution Buffer B | 8 mL |
Bead Tubes | 50 |
Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |