Figure 1. High Quality and Yield of DNA from Saliva Samples. Total DNA was isolated from 250 µL of eight different fresh saliva samples using Norgen's Saliva DNA Isolation Kit Dx (Lanes 1-8). For evaluation, 10 µL of each 100 µL DNA elution was run on a 1.2 % agarose gel. Note the high yield and quality of the DNA in all lanes. Lane M: Norgen's Fast runner 1kb DNA Ladder.
Figure 2. Real-time PCR Consistency from Saliva Samples. Norgen's Saliva DNA Isolation Kit Dx was used to isolate DNA from 250 µL samples of fresh saliva. Ten samples were used from same donor. From the 100 µL elution, 5 µL of saliva DNA was added directly to a real-time PCR master mix (total reaction volume 20 µL), and the real-time PCR reaction was performed. The GAPDH gene was successfully amplified from the different samples without any inhibition and at the same Ct, indicating the isolation consistency and the excellent quality of saliva DNA for downstream applications.
Figure 3. Illumina MiSeq 16s rRNA metagenomics data from saliva samples preserved for over 6 years using Norgen's Saliva DNA Collection and Preservation Devices. The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit Dx (Cat. Dx45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. A) Principal Coordinate Analysis of 12 saliva microbiomes showing differences in the distribution of taxonomic classifications between samples up to kingdom level. B) Hierarchical clustering of 12 saliva microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels. The Proteinase K can be stored at either room temperature or 4oC.
