Direct DNA Extraction Kit (Bacteria)
For research use only and NOT intended for in vitro diagnostics.
Direct DNA Extraction Kit (Bacteria)
Register today to receive an exclusive 15% off* on your first order.
Supporting Data
Figure 1. Detection of Gram negative (E.coli) and Gram positive (Listeria spp.) bacteria using Norgen's Direct DNA Extraction Kit (Bacteria) in real-time PCR system. Two microlitres of the clean supernatant was directly added to a PCR reaction (total 20 µL) to detect 16s rRNA target or Listeria spp. specific gene for E.coli and Listeria spp. respectively. Targets were successfully amplified, indicating the high quality of the inhibitor-free DNA that was extracted using Norgen's Direct DNA Extraction Kit (Bacteria). This kit is applicable for rapid and sensitive microorganism detection for food quality monitoring and other high throughput analysis applications.
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 61500 (50 preps) |
|---|---|
Buffer DL | 2 x 25 mL |
Bead Tubes | 50 |
Product Insert | 1 |
Documentation
FAQs
Bead Tube
If the DNA does not perform well in downstream applications, it may be due to one or more of the following:
- Too much starting material.
Reduce input volume from 1 mL to 0.5 mL or dilute the sample from step 2e in a ratio of 1:10 in Buffer DL.
- PCR inhibitors from cell culture media or food.
If food-related PCR inhibitors or cell culture media interfere with the PCR, the cell pellet can be washed with 400 µL of Buffer DL as described in step 2a.
- PCR reaction conditions need to be optimized.
Take steps to optimize the PCR conditions being used, including varying the amount of template, changing the source of Taq polymerase, looking into the primer design, and adjusting the annealing conditions.