Figure 2. Growth rates of HeLa cells in media containing Exosome-depleted FBS. Growth rates of HeLa cells in media containing Exosome-depleted FBS using Norgen's FBS Exosome Depletion Kits (Slurry Format) was compared to that in media containing standard FBS. Simply, HeLa cells were seeded in DMEM with either 10% Exosome-depleted FBS using Norgen's Kits or 10% standard FBS and then cultured under standard conditions at 37°C with 5% CO² for 3 days. The cells were imaged using Moticam 480 to observe cellular morphology and growth rate. Similar growth and identical cellular morphology were detected for both the Exosome-depleted FBS using Norgen’s FBS Exosome Depletion Kits and the standard FBS

Figure 3. Exosome-depleted FBS with Norgen's FBS Exosome Depletion Kits (Column Format) has undetectable Bovine exosomes levels. Norgen's FBS Exosome Depletion Kit I (Column Format) (Cat# 61200) was used to deplete bovine miRNA from 5mL FBS. Total RNA/miRNA including exosomal RNA was purified from the depleted FBS, non-depleted FBS and a commercially available ready – to – go depleted FBS using Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit (Cat# 55800). Five different bovine microRNAs were assessed by RT-qPCR (miR-26a, miR-30a, miR-92a, miR-23a and miR-122). Three out of the five tested miRNA (miR-26a, miR-92a and miR-23a) didn’t show any amplification in the FBS depleted using Norgen's FBS Exosome Depletion Kit I (Column Format) whereas the other two miRNAs (miR-30a and miR-122) showed very late Ct. values which appeared to be a primer dimer according to the melt curve.