Figure 2. Isolation of Free-Circulating RNA after the isolation of Exosomal RNA from different plasma volumes. Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit (Cat# 59500) was used to isolate Free-Circulating RNA after the isolation of Exosomal RNA from different plasma volumes ranging from 0.1 mL and up to 1 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma free-circulating miR-21. (A) The free-circulating miR-21 is linearly decreasing with increasing the sample input volume. B) The relative amount of the free-circulating miR-21 shows excellent linearity with a percentage of recovery of more than 90%.

Figure 3. Isolation of RNA from exosomes purified from different plasma volumes. Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit (Cat# 59600) was used to isolate Exosomal RNA from different plasma volumes ranging from 1 mL and up to 4 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The plasma exosomal miR-26a is linearly decreasing with increasing the sample input volume. B) The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.

Figure 4. Isolation of Free-Circulating RNA after the isolation of Exosomal RNA from different plasma volumes. Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit (Cat# 59600) was used to isolate Free-Circulating RNA after the isolation of Exosomal RNA from different plasma volumes ranging from 1 mL and up to 4 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma free-circulating miR-21. (A) The free-circulating miR-21 is linearly decreasing with increasing the sample input volume. B) The relative amount of the free-circulating miR-21 shows excellent linearity with a percentage of recovery of more than 90%.

Figure 5. Isolation of RNA from exosomes purified from different plasma volumes. Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit (Cat# 59700) was used to isolate exosomal RNA from different plasma volumes ranging from 4 mL and up to 10 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The plasma exosomal miR-26a is linearly decreasing with increasing the sample input volume. B)The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.

Figure 6. Isolation of Free-Circulating RNA after the isolation of Exosomal RNA from different plasma volumes. Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit (Cat# 59700) was used to isolate free-circulating RNA after the isolation of exosomal RNA from different plasma volumes ranging from 4 mL and up to 10 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma free-circulating miR-21. (A) The free-circulating miR-21 is linearly decreasing with increasing the sample input volume. B) The relative amount of the free-circulating miR-21 shows excellent linearity with a percentage of recovery of more than 90%.