Figure 2. Isolation of Free-Circulating RNA after the isolation of Exosomal RNA from different urine volumes. Norgen’s Urine Exosome and Free-Circulating RNA Isolation Mini Kit (Cat# 59200) was used to isolate Free-Circulating RNA after the isolation of exosomal RNA from different urine volumes ranging from 0.25 mL and up to 1 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary free-circulating miR-21. (A) The free-circulating miR-21 is linearly decreasing with increasing the sample input volume. B) The relative amount of the free-circulating miR-21 shows excellent linearity with a percentage of recovery of more than 90%.

Figure 3. Isolation of RNA from exosomes purified from different urine volumes. Norgen’s Urine Exosome and Free-Circulating RNA Isolation Midi Kit (Cat# 59300) was used to isolate Exosomal RNA from different urine volumes ranging from 2 mL and up to 10 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary exosomal miR-30a. (A) The urinary exosomal miR-30a is linearly decreasing with increasing the sample input volume. B) The relative amount of the urinary exosomal miR-30a shows excellent linearity with a percentage of recovery of more than 90%.

Figure 4. Isolation of Free-Circulating RNA after the isolation of Exosomal RNA from different urine volumes. Norgen’s Urine Exosome and Free-Circulating RNA Isolation Midi Kit (Cat# 59300) was used to isolate Free-Circulating RNA from after the isolation of Exosomal RNA from different urine volumes ranging from 2 mL and up to 10 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary free-circulating miR-21. (A) The free-circulating miR-21 is linearly decreasing with increasing the sample input volume. B) The relative amount of the free-circulating miR-21 shows excellent linearity with a percentage of recovery of more than 90%.

Figure 5. Isolation of RNA from exosomes purified from different urine volumes. Norgen’s Urine Exosome and Free-Circulating RNA Isolation Maxi Kit (Cat# 59400) was used to isolate exosomal RNA from different urine volumes ranging from 11 mL to 30 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary exosomal miR-30a. (A) The urinary exosomal miR-30a is linearly decreasing with increasing the sample input volume. B) The relative amount of the urinary exosomal miR-30a shows excellent linearity with a percentage of recovery of more than 90%.

Figure 6. Isolation of Free-Circulating RNA after the isolation of Exosomal RNA from different urine volumes. Norgen’s Urine Exosome and Free-Circulating RNA Isolation Maxi Kit (Cat# 59400) was used to isolate Free-Circulating RNA after the isolation of exosomal RNA from different urine volumes ranging from 11 mL to 30 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary free-circulating miR-21. (A) The free-circulating miR-21 is linearly decreasing with increasing the sample input volume. B) The relative amount of the free-circulating miR-21 shows excellent linearity with a percentage of recovery of more than 90%.