PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System)
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For research use only and NOT intended for in vitro diagnostics.
PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System)
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Supporting Data
Figure 1. Efficient Clean-Up and Recovery. The performance of Norgen's PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) was compared to Norgen's column-based PCR Purification Kit and Sequencing Reaction Clean-Up Kit by purifying and cleaning 20 µL of 16S NGS library reaction and an end-point PCR. For evaluation, 10 µL of each 50 µL elution were run on a 1X TAE 1.4% agarose gel. As it can be seen, Norgen's PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) was able to successfully recover the target bands without any primer dimer contamination. Marker = Norgen's FastRunner DNA Ladder.
Figure 2. Efficient Clean-Up and Recovery. The performance of Norgen's PCR and Sequencing Reaction Clean-Up 96-Well Kit (Magnetic Bead System) was tested by purifying and cleaning 20 µL of 16S NGS library reaction and an end-point PCR. For evaluation, 10 µL of each 50 µL elution were run on a 1X TAE 1.4% agarose gel. As it can be seen, Norgen's PCR and Sequencing Reaction Clean-Up 96-Well Kit (Magnetic Bead System) was able to successfully recover the target bands without any primer dimer contamination. Marker = Norgen's FastRunner DNA Ladder (Cat. 12800).
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 60200 (50 preps) | Cat. 62700 (192 preps) |
---|---|---|
Binding Buffer C | 30 mL | 2 x 30 mL |
Elution Buffer B | 8 mL | 15 mL |
Magnetic Bead Suspension | 1.1 mL | 8.5 mL |
Elution Tubes (1.7 mL) | 50 | - |
96-Well Elution Plate | - | 2 |
Adhesive Tape | - | 2 |
Product Insert | 1 | 1 |