Name: Low Abundance RNA Quantification Kit
Brand: Norgen Biotek Corp.
SKU: 58900
Price: 335.000000 USD
Availability: InStock

Supporting Data

Figure 1. Sensitivity of RNA Quantification with the Low Abundance RNA Quantification Kit compared to Other Methods. A diagram representing the dynamic range of different RNA quantification method is presented here. The Low Abundance RNA Quantification Kit can quantify purified RNA from low abundance samples in the pg and sub-pg range.

Figure 2. A representative RT-qPCR baseline graph showing the successful amplification of a dilution series of Quantified RNA Standard.

Figure 3. Sensitivity of RNA Quantification from Small Volumes of Human Plasma using the Low Abundance RNA Quantification Kit. A representative qPCR Baseline Graph showing the amplification of total plasma RNA isolated from either 50 or 200 µL of human plasma using Norgen's Plasma/Serum RNA Purification Mini Kit (Cat. 55000). The Low Abundance RNA Quantification Kit could quantify purified RNA from such low abundance samples (and others such as urine, exosomes etc) with purified RNA concentrations that are 100 pg per µL or less.

Figure 4. Sensitive RNA Quantification from Small Volumes of Plasma

Storage Conditions
Upon receipt, store Norgen’s Low Abundance RNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.

Component	Cat. 58900 (48 reactions)
microScript microRNA Enzyme Mix	20 μL
2x microScript Reverse Transcriptase Reaction Mix	0.5 mL
2X Real-Time PCR Master Mix	1 mL
RNA Quantification Primer Set Mix	200 μL
Quantified RNA Standard 50 pg/μL	2 x 60 μL
Nuclease-Free Water	3 x 1.25 mL
Product Insert	1

Low Abundance RNA Quantification Kit