Blood DNA Isolation Kits

Features and Benefits

High yield and high purity DNA ready for any application
Available in a variety of formats to properly suit your needs
Compatible with blood collected on a variety of commercially available tubes

These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.

Blood DNA Isolation Mini Kit

Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.

Blood DNA Isolation Midi Kit

Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.

Blood DNA Isolation Maxi Kit

This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.

Blood DNA Isolation 96-Well Kit (High Throughput)

This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.

Blood DNA Isolation Kit (Magnetic Bead System)

Norgen's Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.

Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

Figure 1. The difference of Ct values obtained from a Taqman® qPCR reaction performed on DNA isolated from blood collected on Citrate, EDTA, and Heparin anticoagulants. 3 and 9 μL of each solution were used in a 20 μL qPCR reaction involving GAPDH primers.

Figure 2. Resolution of DNA isolated from buffy coats using Norgen's Blood Genomic DNA Isolation Mini Kit. 15 μL of 200 μL elutions were run on 1X TAE 1.0% agarose gel. Marker= Norgen's UltraRanger DNA Ladder.

Figure 3. The difference in Ct values between DNA isolated using Norgen's Blood Genomic DNA Isolation Mini Kit (Norgen), Competitor A's DNA Blood Mini Kit (A), Competitor B's Blood Kit (B), and Competitor B's Dx Blood (B Dx), in a Taqman qPCR reaction. Different template volumes (1, 3, 6 & 9 μL) of each elution were used in a 20 μL qPCR reaction involving GAPDH primers.

Figure 4. The difference in yield isolated from different volumes of blood using Norgen's vs Qiagen's kit. 50 μL of each sample was diluted in 450 μL of nuclease-free water, and DNA concentrations were measured using the UltraSpec 2100 Pro (Fisher Scientific). Two elutions were performed for all samples.

Figure 5. High Yields of DNA Isolated from Whole Blood. DNA was isolated from two different 10mL whole blood samples using Norgen's Blood DNA Isolation Maxi Kit. Following isolation, 10 µL from each 1 mL elution was loaded on 1% TAE agarose gel. Lanes 1 and 3 correspond to the first elution, while Lanes 2 and 4 correspond to the second elution. Norgen's Blood DNA Isolation Maxi Kit demonstrated a high yield of intact DNA.

Figure 6. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 10 mL of whole human blood using Norgen's Blood DNA Isolation Maxi Kit. Different input amounts (1, 3 & 6 µL) of the DNA from each of the 1mL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.

Figure 7. High Yields of DNA Isolated from 20 µL to 200 µL of Whole Blood. DNA was isolated from 20, 50, 100 and 200 µL of whole blood using Norgen's Blood DNA Isolation Mini Kit and a leading competitor's kit. Following isolation, 15 µL from each 200 µL elution was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation Mini Kit demonstrated a better DNA yield than the leading competitor's kit. Lane M: Norgen’s UltraRanger 1kb DNA Ladder.

Figure 8. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 20, 50, 100 and 200 µL of whole human blood using Norgen's Blood DNA Isolation Mini Kit (Blue) and a leading competitor's kit (Red). 9 µL of the DNA from each 200 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value, indicating the higher yield and purity of DNA isolated using Norgen's kit. The black line is a no-template control.

Figure 9. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 20, 50, 100 and 200 µL of whole human blood using Norgen's Blood DNA Isolation Mini Kit (Blue) and a leading competitor's kit (Red). Nine µL of the DNA from each 200 µL of elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, with a linear decrease in Ct value with the increase in blood input volume, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value from all DNA isolated from the different blood input volumes, indicating the higher yield and purity of DNA isolated using Norgen's kit.

Figure 10. High Yields of DNA Isolated from 2 mL of Whole Blood. DNA was isolated from 2 mL of whole blood using Norgen's Blood DNA Isolation Midi Kit. Following isolation, 20 µL from 300 µL first and second elutions was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation Midi Kit demonstrated a high DNA yield and integrity. Lane L: Norgen's HighRanger 1kb DNA Ladder.

Figure 11. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 2 mL of whole human blood using Norgen's Blood DNA Isolation Midi Kit. Different input amounts (1, 3 & 6 µL) of the DNA from each of the 300 µL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.

Figure 12. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 2 mL of whole human blood using Norgen's Blood DNA Isolation Midi Kit. Different input amounts (1, 3 & 6 µL) of the DNA from each of the 300 µL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene with a linear decrease in Ct value with the increased DNA template volume, indicating that the DNA is of a high quality and can be used in sensitive downstream applications.

Figure 13. High Yields of DNA Isolated from 200 µL of Whole Blood. DNA was isolated from 200 µL of whole blood using Norgen's Blood DNA Isolation 96-Well Kit. Following isolation of 12 samples, 15 µL from each 200 µL elution was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation 96-Well Kit demonstrated a good and consistent DNA yield and integrity. Lane M: Norgen's HighRanger 1kb DNA Ladder.

Figure 14. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 200 µL of whole human blood using Norgen's Blood DNA Isolation 96-Well Kit. Five µL of the DNA from each 200 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene from all the isolated 12 samples (blue). This indicates that the isolated DNA from all samples is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.

Figure 15. DNA Isolated from Blood Preserved in 3 Different Anticoagulants. DNA was isolated from 200 µL of human whole blood samples preserved in three different anticoagulants (Heparin: H, EDTA: E and Na Citrate: C) using Norgen's Blood DNA Isolation Kit (Magnetic Bead System) and Norgen's Blood DNA Isolation Mini Kit (column format, Cat. 46300). For evaluation, 10 µL from each 200 µL of elution were run on 1X TAE 1.2% agarose gel. Norgen's Blood DNA Isolation Kit (Magnetic Bead System) showed an intact and comparable DNA profile to the column method from all three different anticoagulant mixed blood samples. Marker = Norgen's HighRanger DNA Ladder.

Figure 16. High Yield of Blood DNA. DNA was isolated from 200 µL of human whole blood samples preserved in three different anticoagulants (Heparin, EDTA and Na Citrate) using Norgen's Blood DNA Isolation Kit (Magnetic Bead System) and Norgen's Blood DNA Isolation Mini Kit (column format, Cat. 46300). The total DNA yield from all the elutions was compared, and it was found that the Magnetic Bead System isolated a similar DNA yield to the column method from all the different anticoagulants tested.

Figure 17. Detection of GAPDH from blood samples preserved in three different anticoagulants (Heparin: red, EDTA: green and Na Citrate: blue). DNA was isolated from 200 µL human whole blood samples preserved in three different anticoagulants using Norgen's Blood DNA Isolation Kit (Magnetic Bead System) and Norgen's Blood DNA Isolation Mini Kit (column format, Cat. 46300). The high quality of the purified DNA was confirmed by Real-time PCR using 2 µL (circle), 4 µL (triangle) and 8 µL (cross) (total PCR reaction volume was 20 µL). No PCR inhibition was observed up to 8 µL of PCR template, indicating the excellent quality of blood DNA isolated using Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) similar to the column method (Cat. 46300).

Figure 18. DNA Isolated from Blood Preserved in EDTA Anticoagulants. DNA was isolated from 200 µL of human whole blood samples using Norgen's Blood DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL from each 200 µL of elution were run on 1X TAE 1.2% agarose gel. Norgen's Blood DNA Isolation 96-Well Kit (Magnetic Bead System) showed an intact DNA profile. Marker = Norgen's HighRanger DNA Ladder (Cat. 11900).

Figure 19. Detection of GAPDH From Blood Samples Preserved in EDTA Anticoagulants. DNA was isolated from 200 µL human whole blood samples using Norgen's Blood DNA Isolation 96-Well Kit (Magnetic Bead System). The high quality of the purified DNA was confirmed by Real-time PCR using 2 µL of purified DNA in a total PCR reaction volume of 20 µL. No PCR inhibition was observed, indicating the excellent quality of blood DNA isolated using Norgen’s Blood DNA Isolation 96-Well Kit (Magnetic Bead System).

Kit Specifications
Minimum Blood Input	20 µL
Maximum Blood Input	200 µL
Column Binding Capacity	> 50 µg
Average Yield (200 µL of blood)	4-12 µg*
Time to Complete 10 Purifications	30 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 46300 (50 preps)	Cat. 46380 (100 preps)	Cat. 51400 (20 preps)	Cat. 31200 (12 preps)	Cat. 46350 (192 preps)	Cat. 59800 (50 preps)	Cat. 62600 (192 preps)
Lysis Buffer B	20 mL	2 x 20 mL	2 x 40 mL	2 x 110 mL	2 x 40 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	2 x 18 mL	55 mL	55 mL	55 mL	18 mL	55 mL
Wash Solution A	18 mL	2 x 18 mL	2 x 38 mL	2 x 38 mL	2 x 38 mL	-	-
Elution Buffer B	30 mL	2 x 30 mL	30 mL	30 mL	2 x 30 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K in Storage Buffer	1.2 mL	2 x 1.2 mL	4.4 mL	8 mL	4.5 mL	1.2 mL	4 mL
Magnetic Bead Suspension	-	-	-	-	-	2 x 1.1 mL	8.5 mL
Maxi Spin Columns	-	-	-	12	-	-	-
Mini Spin Columns	50	100	-	-	-	-	-
Midi Spin Columns	-	-	20	-	-	-	-
96-Well Plate	-	-	-	-	2	-	2
Adhesive Tape	-	-	-	-	4	-	2
Collection Tubes	50	100	20	12	-	-	-
96-Well Collection Plate	-	-	-	-	2	-	-
Elution Tubes	50	100	20	12	-	50	-
96-Well Elution Plate	-	-	-	-	2	-	2
Product Insert	1	1	1	1	1	1	1

Documentation

FLYERS

Flyer

Citations

Title	Genetic differentiation and inheritance of randon amplified polymorphic DNA (RAPD) markers in pectoral spine phenotypic sub-groups of Clarias gariepinus
Citation	African Journal of Biotechnology 2013.
Authors	OO Oyebola, BO Omitoyin, AE Salako, EK Ajani, MO Awodiran

Title	Non Genetic Risk Factor and Polymorphism of Aldosterone Synthase T(-344)C Variant CYP11B2 Gene Promoter Region Essential Hypertension Patients at Coastal and Mountainous Area
Citation	Jurnal Kedokteran Brawijaya 2013.
Authors	Sundari, D. Wahono, M. A. Widodo