Figure 2. Two of Norgen’s soil DNA isolation methods (Column vs Magnetic Bead System) were compared for DNA concentration. All DNA elutions isolated using Norgen’s Soil DNA Isolation Kit (Magnetic Bead System) [A to E] showed a comparable DNA concentration to Norgen’s Soil DNA Isolation Kit (Column Method; Cat. 26500), indicating the consistent and robust performance of the Soil DNA Isolation Kit (Magnetic Bead System).

Figure 3. High soil DNA quality was confirmed by real-time PCR using 8 µL of soil DNA (total PCR reaction volume was 20 µL) isolated using Norgen’s Soil DNA Isolation Kit (Magnetic Bead System) to detect 16s rDNA. PCR results from Norgen's Soil DNA Isolation Kit (Magnetic Bead System) (circle) were comparable to Norgen’s column based Soil DNA Isolation Kit (cross) and showed the successful 16s rDNA detection without PCR inhibition, indicating the excellent soil DNA quality using Norgen’s Soil DNA Isolation Kit (Magnetic Bead System).

Figure 4. Hierarchical Clustering Dendrogram. High quality of soil DNA was successfully isolated from the 5 challenging soil types using Norgen's Soil DNA Isolation Kits (A: Soil DNA Isolation Kit (Magnetic Bead System) and B: Soil DNA Isolation Kit Spin Column) respectively. The Hierarchical Clustering Dendrogram is based on genus-level classifications and shows the relative abundance of its genus-level classifications among soil types.

Figure 5. Principal Coordinate Analysis (PCoA) generated by Illumina MiSeq. Principal Coordinate Analysis of 20 samples (25-28: Mulch, 29-32: Clay, 33-36: Potting soil, 76-78: Top soil, 79-82: Compost) showing differences in the distribution of taxonomic classifications between samples up to species level. Each soil type is clearly clustered together and away from other types.

Figure 6. Resolution of DNA Isolated from Top Soil. DNA was isolated from high humic acid soil (top soil) using Norgen's Soil DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL from the 75 µL elution was run on 1X TAE 1.2% agarose gel. Excellent DNA integrity, consistency and yield were observed from the Soil DNA Isolation 96-Well Kit (Magnetic Bead System). Marker = Norgen’s HighRanger DNA Ladder (Cat. 11900).

Figure 7. High Soil DNA Quality Was Confirmed By Real-time PCR Using 2 µL of Soil DNA (Total PCR Reaction Volume Was 20 µL) Isolated Using Norgen’s Soil DNA Isolation 96-Well Kit (Magnetic Bead System) to Detect 16s rRNA. PCR results from Norgen's Soil DNA Isolation 96-Well Kit (Magnetic Bead System) showed the successful 16s rRNA detection without PCR inhibition, indicating the excellent soil DNA quality using Norgen’s Soil DNA Isolation 96-Well Kit (Magnetic Bead System).