Name: Saliva Exosome Purification Kit
Brand: Norgen Biotek Corp.
SKU: 65300
Price: 819.000000 USD
Availability: InStock

Features and Benefits

Versatile sample input ranging from 0.5 mL to 2 mL
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents or overnight incubation required
No protease treatment required
Purify pure exosomes that are free from RNA-binding proteins
Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
Process fresh saliva or saliva collected with Norgen's Saliva Exosome Collection and Preservation Kit (Cat 65400).
Isolate high quality RNA from purified exosomes using Norgen's Exosome RNA Purification Kit (Cat. 58000)
Purification is based on Norgen’s proprietary resin

Norgen’s Saliva Exosome Purification Kit allows for the purification of saliva exosomes from 500 μL to 2 mL of fresh saliva samples or saliva samples collected using Norgen's Saliva Exosome Collection and Preservation Kit (Cat 65400). This kit also allows for the purification of intact extracellular vesicles (EVs) from different saliva sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal transcripts.

Details

Kit Specifications
Saliva Input	500μL - 2mL
Size of Exosome Purified	40nm - 150nm
Elution Volume	200μL
Time to Complete 10 Purifications	15 - 30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat No. 65300 (50 preps)
Slurry E	12.5 mL
ExoC Buffer	1.5 mL
ExoR Buffer	12 mL
Mini Filter Spin Columns inserted into 2 mL tubes	50
Product Insert	1

Documentation

PROTOCOLS

(65300) Saliva Exosome Purification Kit - Protocol

SAFETY DATA SHEETS

65300 - Saliva Exosome Purification Kit - SDS

RELATED BLOGS

FAQs

Spin Column

What should I do if some of the grey resin is transferred out when I am decanting the saliva supernatant?

Simply remix and recentrifuge. After centrifuging decant the supernatant

What if I added more or less of Slurry E?

Adding less volume may reduce the amount of the purified exosomes. Adding more may not affect the exosome capture but may affect the release of the purified exosomes in the ExoR Buffer.

What if I added more or less of ExoC Buffer?

Adding a different volume from the specified optimum volume will significantly reduce the amount of the purified exosomes.

What if I added more or less of ExoR Buffer?

Adding less volume will reduce the release of the captured exosomes in the ExoR Buffer. Adding more will not affect the release of the captured exosomes but it will be more diluted.

What will happen if accidently some of the grey resin was transferred with the ExoR buffer?

Any grey resin will be filtered through the Mini Filter Spin Column and the flowthrough which contains the purified exosomes should not contain any grey resin.

Is ExoR buffer from Norgen's exosome isolation kit compatible with proteomic downstream applications?

ExoR buffer is compatible with most proteomic downstream applications. The ExoR buffer doesn't contain any detergents. Please contact our Tech support team at support@norgenbiotek.com and ask for reference publications.

How to store the purified exosomes?

If not using for downstream application immediately, it is recommended to store the purified exosomes at -80 degrees.

Are exosomes purified using Norgen Biotek's technology biology active?

Yes, the exosomes purified using Norgen Biotek's technology are biology active. Please contact our Tech support team at support@norgenbiotek.com and ask for reference publications.

What should I do if some of the grey resin is transferred out when I am decanting the saliva supernatant?

Simply remix and recentrifuge. After centrifuging decant the supernatant.

What if I added more or less of Slurry E?

Adding less volume may reduce the amount of the purified exosomes. Adding more may not affect the exosome capture but may affect the release of the purified exosomes in the ExoR Buffer.

What if I added more or less of ExoC Buffer?

Adding a different volume from the specified optimum volume will significantly reduce the amount of the purified exosomes.

What if I added more or less of ExoR Buffer?

Adding less volume will reduce the release of the captured exosomes in the ExoR Buffer. Adding more will not affect the release of the captured exosomes but it will be more diluted.

What will happen if accidently some of the grey resin was transferred with the ExoR buffer?

Any grey resin will be filtered through the Mini Filter Spin Column and the flowthrough which contains the purified exosomes should not contain any grey resin.

Is ExoR buffer from Norgen's exosome isolation kit compatible with proteomic downstream applications?

The ExoR buffer doesn't contain any detergents, and is compatible with most of proteomic downstream applications. Please contact our technical support team at support@norgenbiotek.com and ask for reference publications.

How to store/preserve the purified exosomes?

We recommend storing the isolated Exosomes at -80 degrees and to avoid multiple freeze-thaw cycles.

Citations

Title	The emerging role of small extracellular vesicles in saliva and gingival crevicular fluid as diagnostics for periodontitis
Citation	Journal of periodontal research 2022.
Authors	Han P, Bartold PM, Ivanovski S.

Saliva Exosome Purification Kit