PCR Sizer 100 bp DNA Ladder
Ideal for PCR product size confirmation
For research use only and NOT intended for in vitro diagnostics.
PCR Sizer 100 bp DNA Ladder
Ideal for PCR product size confirmation
$75.00
SKU
11400
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Features and Benefits
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Ten discrete fragments from 100 bp to 1000 bp
- Higher intensity reference band at 500 bp
The Norgen PCRSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCRSizer contains ten discrete fragments ranging from 100 bp to 1000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for PCR product size confirmation.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
PCR Sizer 100 bp DNA Ladder (Cat# 11400) - 100 loads
Ladder Properties:
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
1000
|
78
|
|
2
|
900
|
70
|
|
3
|
800
|
62
|
|
4
|
700
|
55
|
|
5
|
600
|
47
|
|
6
|
500
|
78
|
|
7
|
400
|
31
|
|
8
|
300
|
24
|
|
9
|
200
|
31
|
|
10
|
100
|
24
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
Citations
| Title | Occurrence of Hepatitis A Virus, Rotavirus and Astrovirus in Arca noae and Shrimp Samples Collected from Damanhur City, Egypt |
| Citation | Egyptian Journal of Aquatic Biology & Fisheries 2024. |
| Authors | Sahar Abd Al-Daim |
| Title | One-Year Investigation of Hepatitis A Virus and Rotavirus in Two Waste Water Treatment Plants in Egypt |
| Citation | Journal of Biomedical Research and Environmental Sciences 2024. |
| Authors | Sahar Abd Al-Daim |
| Title | stribution Profile of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli Isolates from Asa River (Nigeria) |
| Citation | Journal of Applied Sciences and Environmental Management 2017. |
| Authors | Olatunji, M. K., Olayemi, A., & Jimoh, F |
| Title | Food control and a citizen science approach for improving teaching of Genetics in universities |
| Citation | IUBMB Journals 2016. |
| Authors | Borrell, Y. J., Muñoz-Colmenero, A. M., Dopico, E., Miralles, L., & Garcia-Vazquez, E |
| Title | Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil |
| Citation | Pesq. agropec. bras., Brasília 2016. |
| Authors | Rivas, E. B., de Almeida Bôdi, E. C., Harakava, R., Gregori, F., & Gonçalves, M. C |
| Title | Standardization of a molecular method for epidemiologic identification of Leishmania strains |
| Citation | Genet. Mol. Res 2016. |
| Authors | Rocha, R. F., Menezes, E. V., Xavier, A. R. E. O., Royo, V. A., Oliveira, D. A., Júnior, A. F. M., ... & Michalsky, E. M |
| Title | Escherchia coli from Crohn's disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteraggregative (EAEC) pathotypes |
| Citation | Gut Pathogen 2015. |
| Authors | AC da Silva Santos, FG Romeiro, LY Sassaki, J Rodrigues |
| Title | EVIDENCE OF HELMINTH INFECTION IN GUANCHE MUMMIES: INTEGRATING PALEOPARASITOLOGICAL AND PALEOGENETIC INVESTIGATIONS |
| Citation | Journal of Parasitology 2015. |
| Authors | Alena Mayo Iniguez, Herminia Gijón-Botella, Lauren Jaeger, MarÃadel Carmen del Arco-Aguilar, Mercedesdel Arco-Aguilar del Arco-Aguilar, Adauto Araujo, Conrado RodrÃguez-Maffiotte, and Mercedes MartÃn-Oval |
| Title | Pathogenesis of acute and chronic diseases caused by cyprinid herpes virus-3 |
| Citation | Journal of Fish Diseases 2014. |
| Authors | S Miwa, I Kiryu, K Ysasa, T Ito, T Kaneko |
| Title | A comparison of microbiological and molecular dertection of vaginal Lactobacillus spp. Between mares and women |
| Citation | Animal Reproduction 2013. |
| Authors | M Castro-Chaves, AS Borges, JP Oliveira-Filho, EM Brolazo, C Ramires-Neto, MA Alvarenga |