PCR Ranger 100 bp DNA Ladder
Ideal for assessment of a range of PCR product sizes
For research use only and NOT intended for in vitro diagnostics.
PCR Ranger 100 bp DNA Ladder
Ideal for assessment of a range of PCR product sizes
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Features and Benefits
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Eleven discrete fragments ranging from 50 bp to 1000 bp
- Higher intensity reference band at 500 bp
The Norgen PCR Ranger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCR Ranger DNA Ladder contains eleven discrete fragments ranging from 50 bp to 1000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for assessment of a range of PCR product sizes.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
PCR Ranger 100 bp DNA Ladder (Cat# 11300) - 100 loads
Ladder Properties:
• Eleven discrete bands, ranging from 50 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
1000
|
91
|
|
2
|
900
|
66
|
|
3
|
800
|
61
|
|
4
|
700
|
51
|
|
5
|
600
|
43
|
|
6
|
500
|
70
|
|
7
|
400
|
28
|
|
8
|
300
|
23
|
|
9
|
200
|
30
|
|
10
|
100
|
22
|
|
11
|
50
|
15
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
Citations
| Title | Characterization and molecular epidemiology of extensively prevalent nosocomial isolates of drug-resistant Acinetobacter spp |
| Citation | Genetics and Molecular Research 2016. |
| Authors | Carvalho, A. A., Cardoso, L. L., Nogueira, H. S., Menezes, E. V., Xavier, M. A. S., Barreto, N. A. P., ... & Xavier, A. R. E. O |
| Title | Combined Exclusion Probabilities of Ten Microsatellite Markers used with Nigerian Chicken Populations |
| Citation | European International Journal of Science and Technology 2016. |
| Authors | Olowofeso, O., Wheto, M., Durosaro, S. O., Bankole, K. O., Adepoju, D. A., & Folarin, O. V |