Features and Benefits

Mycobacterium tuberculosis is a pathogenic bacterial species belonging to the genus Mycobacterium, and is the causative agent of tuberculosis. Tuberculosis (TB) is a multifaceted disease and challenging public health problem in both industrialized and developing countries. According to the WHO, 8.8 million active cases of TB are diagnosed each year and of these, almost 2 million die. Once thought to be under control or even close to extinction, TB infection levels are rising and the threat is compounded by new, virulent and drug-resistant strains. Although most cases occur in the developing world (22 countries accounting for 80% of all global cases), increasing population mobility combined with facility of transmission means that no country is immune from the resurgence of TB. TB control programs are currently facing a number of constraints. Worldwide, fewer than 25% of all tuberculosis cases are detected. Of utmost concern is the absence of a timely and accurate test for the diagnosis of mycobacterial disease. Early diagnosis is crucial for the prevention of further spread of the disease.

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Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

Figure 4. Analytical Sensitivity. The analytical sensitivity of the Positive Control is evaluated through the amplification of a dilution series of the Positive Control Dx (provided, 200,000 copies/μL).

Figure 5. Quantitative Analysis. The Ct values obtained from the amplification curves of the Positive Control were used to generate a standard curve that illustrates the linear relationship between the log Positive Control copy number (Log Starting Quantity, X axis) and the Ct value for each concentration (Cq, Y axis).

Figure 6. Qualitative Analysis. Amplification curve of TaqMan PCR positive results. Effective results are demonstrated by the amplification of both Positive Control (circle) and Internal Control (square) above the baseline for both FAM (red) and HEX (purple) channels.

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Documentation

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