MidRanger 1 kb DNA Ladder
Ideal for sizing larger PCR products (>1kb) and most cloning applications.
For research use only and NOT intended for in vitro diagnostics.
MidRanger 1 kb DNA Ladder
Ideal for sizing larger PCR products (>1kb) and most cloning applications.
$75.00
SKU
11700
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Features and Benefits
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Ten discrete fragments ranging from 300 bp to 5000 bp
The Norgen MidRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our MidRanger contains ten discrete fragments ranging from 300 bp to 5000 bp. This Ladder is ideal for sizing larger PCR products (>1kb) and most cloning applications.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
MidRanger 1kb DNA Ladder (Cat# 11700) - 100 loads
Ladder Properties:
• Ten discrete fragments ranging from 300 bp to 5000 bp
• Ten discrete fragments ranging from 300 bp to 5000 bp
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
5000
|
88
|
|
2
|
4000
|
78
|
|
3
|
3000
|
67
|
|
4
|
2500
|
58
|
|
5
|
2000
|
50
|
|
6
|
1500
|
43
|
|
7
|
1000
|
25 |
|
8
|
700
|
34
|
|
9
|
500
|
33
|
|
10
|
300
|
24
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
Citations
| Title | Detection and Genotyping of Helicobacter pylori among Gastric ulcer and Cancer Patients from Saudi Arabia |
| Citation | Pakistan Journal of Medical Sciences 2017. |
| Authors | Bibi, F., Alvi, S. A., Sawan, S. A., Yasir, M., Sawan, A., Jiman-Fatani, A. A., & Azhar, E. I. (2017). |
| Title | Genetic variation and its reflection on post translational modifications in frequency clock and mating type a-1 proteins in Sordaria fimicola |
| Citation | BioMed Research International 2017. |
| Authors | Arif, R., Akram, F., Jamil, T., Mukhtar, H., & Saleem, M. |
| Title | Detection of DNA methylation pattern in thidiazuron-induced blueberry callus using methylation-sensitive amplification polymorphism |
| Citation | Biologia Plantarum 2016. |
| Authors | Ghosh, A., Igamberdiev, A. U., & Debnath, S. C |