HighRanger Plus 100 bp DNA Ladder
Ideal for size determination of digested DNA
For research use only and NOT intended for in vitro diagnostics.
HighRanger Plus 100 bp DNA Ladder
Ideal for size determination of digested DNA
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Features and Benefits
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Nineteen discrete fragments ranging from 100 bp to 10000 bp
- Higher intensity reference bands at 500 bp, 1000 bp and 5000 bp
The Norgen HighRangerPlus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our HighRangerPlus contains nineteen discrete fragments ranging from 100 bp to 10000 bp with higher intensity reference bands at 500 bp, 1000 bp and 5000 bp. This Ladder is ideal for size determination of digested DNA.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
HighRanger Plus 100 bp DNA Ladder (Cat# 12000) - 100 loads
Ladder Properties:
- Nineteen discrete fragments ranging from 100 bp to 10000 bp
- Higher intensity bands at 500 bp, 1000 bp and 5000 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
10000
|
42
|
|
2
|
8000
|
33
|
|
3
|
6000
|
25
|
|
4
|
5000
|
42
|
|
5
|
4000
|
35
|
|
6
|
3000
|
27
|
|
7
|
2500
|
22
|
|
8
|
2000
|
18
|
|
9
|
1500
|
13
|
|
10
|
1000
|
40
|
|
11
|
900
|
28
|
| 12 | 800 |
25 |
| 13 | 700 |
34 |
| 14 | 600 |
19 |
| 15 | 500 |
44 |
| 16 | 400 |
13 |
| 17 | 300 |
18 |
| 18 | 200 |
13 |
| 19 | 100 |
10 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
This kit is stable for 2 years after the date of shipment.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
Citations
| Title | A new microsporidium species, Nucleospora braziliensis n. Sp. infecting Nile tilapia (Oreochromis niloticus) from brazilian aquaculture |
| Citation | International Journal of Fisheries and Aquatic Studies 2017. |
| Authors | Rodrigues, M. V., Francisco, C. J., Silva, G., David, R. J. D. S., & Júnior, J. P. A. (2017) |
| Title | Gender Identification by Calls and Body Size of the Streaked Shearwater Examined by CHD Genes |
| Citation | Ornithological Science 2014. |
| Authors | H Arima, N Oka, Y Baba, H Sugawa, T Ota |