Features and Benefits

Norgen's Direct DNA Extraction Kit (Bacteria) contains a Direct Pathogen Extraction Buffer (Buffer DL) and pre-filled bead tubes for the extraction of DNA from enriched Gram negative and positive bacteria from food and culture samples. The kit first allows for the extraction of DNA directly from bacteria using mechanical homogenization with Buffer DL. The cell debris is then removed by a short centrifugation and the clean supernatant is ready for use in PCR reactions. Buffer DL is designed to stabilize extracted DNA and inactivate PCR inhibitors to provide a rapid and economic solution for high throughput pathogen detection, particularly for Gram-positive bacteria such as Listeria spp. Norgen's Direct DNA Extraction Kit (Bacteria) is compatible with sensitive detection applications including end-point PCR and real-time PCR.

Direct DNA Extraction Kit (Bacteria)
Procedure

1. Take 1 mL from enriched culture
 

 

2. Pellet the cells and resuspend with 400 µL of Buffer DL

 

3. Homogenize in a bead beater

 

4. Remove the cell debris by centrifugation

 

5. The clean supernatant can be used directly in PCR

Details

Supporting Data

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Figure 1. Detection of Gram negative (E.coli) and Gram positive (Listeria spp.) bacteria using Norgen's Direct DNA Extraction Kit (Bacteria) in real-time PCR system. Two microlitres of the clean supernatant was directly added to a  PCR reaction (total 20 µL) to detect 16s rRNA target or Listeria spp. specific gene for E.coli and Listeria spp. respectively. Targets were successfully amplified, indicating the high quality of the inhibitor-free DNA that was extracted using Norgen's Direct DNA Extraction Kit (Bacteria). This kit is applicable for rapid and sensitive microorganism detection for food quality monitoring and other high throughput analysis applications.  

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